Kev Npaj - Dissolve eosin hauv dej thiab tom qab ntawd ntxiv qhov no rau 95% cawv (ib feem eosin tov nrog 4 ntu cawv). Rau qhov kawg sib tov ntxiv ob peb tee ntawm acetic acid (0.4ml). Cov acetic acid ua kom cov staining siv eosin.
Koj npaj li cas hematoxylin thiab eosin stain?
Nyob hauv ntu nram qab no, cov kauj ruam yooj yim hauv kev ua H&E stain tau piav qhia
- Txhob Cia Siab. …
- Hydrate the Section. …
- Thov cov Hematoxylin Nuclear Stain. …
- Ua tiav Nuclear Stain los ntawm "Blueing" …
- Tshem tawm Cov Kab Ntsig Tom Qab (Differentiate) …
- Thov rau Eosin Counterstain.
Yuav ua li cas dilute eosin stain?
Dilute Eosin stock solution 1:1 nrog 70% ethanol, ces ntxiv 2-3 tee ntawm glacial acetic acid. Staining.
eosin yog dab tsi?
A liab crystalline dye muaj li ntawm cov Potassium, Sodium, los yog Lead ntsev ntawm tetrabromofluorescein. Thawj zaug pom los ntawm Caro hauv xyoo 1871, eosin feem ntau yog siv los ua cov kua qaub rau cov xim liab hauv Silk, Wool, Paper, Leather, thiab Paj Rwb.
Puas eosin stain Basic?
Eosin yog ib qho acidic dye: nws tsis raug nqi (cov qauv dav dav rau acidic dyes yog: Na+dye-). Nws stains yooj yim (lossis acidophilic) qauv liab lossis liab. Qhov no kuj qee zaum hu ua 'eosinophilic'.