txheej txheem ntawm Acid-Fast Stain
- Npaj cov kab mob smear rau ntawm qhov huv thiab tsis muaj roj swb, siv cov txheej txheem tsis muaj menyuam.
- Cia smear rau cua kom qhuav thiab tom qab ntawd kho cua sov. …
- Cov smear nrog carbol fuchsin stain.
- Cua qhov stain kom txog thaum vapor pib nce (piv txwv li txog 60 C). …
- Nyob qhov stain nrog dej huv.
Cov kab mob acid-ceev stained li cas?
Vim tias cov phab ntsa ntawm tes tiv taus ntau cov tebchaw, cov kab mob acid-ceev ceev yuav tsum muaj cov txheej txheem tshwj xeeb staining. Thawj cov stain siv hauv cov kua qaub-ceev staining, carbolfuchsin, yog lipid-soluble thiab muaj phenol, uas pab cov stain nkag mus rau ntawm phab ntsa ntawm tes. Qhov no yog pab ntxiv los ntawm kev sib ntxiv ntawm cov cua sov.
Qhov twg siv rau AFB?
Tus Ziehl Neelsen (ZN) txoj kev ntawm staining acid ceev bacilli tau nyob rau hauv vogue rau ntau tshaj puas xyoo. Nyob rau hauv txoj kev ZN [1], qhov yooj yim fuchsin phenol dye yog siv kub nyob rau ntawd los ntawm melting lub unsaponifiable waxy tshuaj nyob rau saum npoo ntawm lub cell phab ntsa.
3 kauj ruam tseem ceeb ntawm cov kua qaub nrawm yog dab tsi?
Acid-Fast Staining Instructions
- Cua qhuav thiab cua sov kho cov yeeb yaj kiab nyias ntawm cov kab mob. …
- Dej nyab lub swb nrog Carbolfuchsin. …
- Dej swb nrog Acid Cawv rau 30 vib nas this. …
- Counterstain los ntawm dej nyab lub swb nrog Methylene Blue rau 30 vib nas this. …
- Qhia qhov swb los ntawm kev tso nwsnruab nrab ntawm nplooj ntawv ntawm ib phau ntawv Bibulous.
Vim li cas carbol Fuchsin siv rau hauv cov kua qaub nrawm?
Nws yog feem ntau siv rau hauv cov kab mob ntawm cov kab mob mycobacteria vim nws muaj kev sib raug zoo rau cov mycolic acids pom hauv lawv cov cell membranes. … Carbol fuchsin yog siv los ua thawj stain dye txhawm rau kuaj cov kab mob acid-ceev vim tias nws yog soluble hauv cov cell phab ntsa lipids ntau dua li cov kua qaub acid.
