Nyob hauv Ziehl–Neelsen stain, heat ua lub cev mordant thaum phenol (carbol carbol Carbol fuchsin, carbol-fuchsin, lossis carbolfuchsin, yog Kev sib xyaw ntawm phenol thiab yooj yim fuchsin, siv rau hauv cov kab mob staining cov txheej txheem. … Yog tias cov kab mob acid-ceev cov kab mob yuav khaws cov xim liab ntawm cov xim vim lawv muaj peev xwm tiv taus cov kua qaub cawv (0.4 -1% HCl hauv 70% EtOH). https://en.wikipedia.org › wiki › Carbol_fuchsin
Carbol fuchsin - Wikipedia
ntawm carbol fuschin) ua raws li cov tshuaj mordant. Txij li thaum Kinyoun stain yog ib txoj kev txias (tsis muaj cua sov), qhov concentration ntawm carbol fuschin siv yog nce.
Dab tsi yog mordant hauv acid ceev staining?
Thaum lub sijhawm cov kua qaub nrawm nrawm, heatyog siv los ua cov tshuaj mordant kom tso cai rau cov stains nkag mus rau hauv cov quav ciab mycolic acid txheej. Cov cua sov yuav tiv thaiv cov hlwb los ntawm kev destained siv cov kua qaub-cawv. Vim tias cov hlwb no tuav ceev ceev mus rau thawj qhov stain nrog cov kua qaub cawv, lawv hu ua kua qaub ceev zoo.
Dab tsi yog mordant siv hauv Ziehl-neelsen txoj kev staining rau AFB?
Franz Ziehl tom qab ntawd hloov Ehrlich cov txheej txheem staining los ntawm kev siv carbolic acid ua mordant. Friedrich Neelsen khaws Ziehl txoj kev xaiv ntawm mordant tab sis hloov thawj qhov stain rau carbol fuchsin.
Lub hauv paus ntsiab lus ntawm cov kua qaub ceev yog dab tsiZiehl-neelsen txheej txheem?
PURPOSE: Siv rau hauv kev ua qauv qhia ntawm cov kab mob acid-ceev ceev uas yog cov genus 'mycobacterium', uas suav nrog cov kab mob ua rau tuberculosis. TSEEM CEEB: Lub lipoid capsule ntawm cov kab mob acid-ceev ceev yuav siv carbol- fuchsin thiab tiv taus decolorization nrog dilute acid yaug.
Lub hauv paus ntsiab lus ntawm acid-ceev bacilli yog dab tsi?
Cov phab ntsa ntawm cov kab mob mycobacterial muaj mycolic acids, uas yog fatty acids uas ua rau cov yam ntxwv ntawm "acid-fastness." Lub hauv paus ntsiab lus ntawm AFB smear yog raws li qhov tseeb tias mycolic acid nyob rau hauv lub cell phab ntsa ntawm AFB ua rau lawv tiv taus decolorization nrog acid cawv.
