Cov kab zauv Scatchard yog ib qho kev sib npaug siv hauv molecular biology los xam cov affinity thiab tus naj npawb ntawm cov chaw sib txuas ntawm cov receptor rau ib lub ligand. Nws muaj npe tom qab American chemist George Scatchard.
Daim phiaj xwm Scatchard qhia koj li cas?
Cov phiaj xwm Scatchard feem ntau yog siv los txiav txim siab qhov kev sib raug zoo ntawm cov receptor rau nws cov ligand thiab cov naj npawb ntawm cov chaw sib khi ; qhov titration nkhaus zoo tshaj plaws qhia tau hais tias qhov kev sib raug zoo yog txiav txim los ntawm cov ntsiab lus saum toj no thiab hauv qab Kd, thiab qhia tag nrho cov lus teb; Lub Hill Plot feem ntau yog siv los txiav txim siab kev koom tes…
BMAX Scatchard plot yog dab tsi?
Bmax is the X intercept ; Kd yog qhov tsis zoo reciprocal ntawm txoj kab nqes. Thaum ua Scatchard zaj duab xis, koj yuav tsum xaiv chav rau Y axis. … Thaum cov txiaj ntsig no nyuaj los txhais, lawv yooj yim suav ntawm Kd uas sib npaug ntawm qhov sib npaug ntawm txoj kab nqes.
Dab tsi yog qhov tsis yog kab lus Scatchard txhais li cas?
Nonlinear Scatchard cov phiaj xwm yog kuaj mob ntawm ntau yam nyuaj ntawm ligand-receptor kev sib cuam tshuam. Ib zajlus uas yog concave downward qhia qhov zoo homotropic kev koom tes ntawm binding qhov chaw ntawm ib tug allosteric receptor. … Lub abscissa cuam tshuam dua qhov sib npaug ntawm cov chaw sib khi ntau tshaj plaws.
Koj ua li cas txiav txim siab tus lej ntawm qhov chaw khi ntawm Scatchard daim phiaj?
By plotting n/[L] tiv n, Scatchard zaj lus qhia tias nqes hav sib npaug rau-1/Kd thaum lub x-intercept sib npaug ntawm cov ligand khi qhov chaw n.
