lim. (biochemistry) Kev qhia ntawm ib qho enzyme uas yog lub luag haujlwm rau cov metabolism ntawm tus neeg sawv cev qhia nws.
autoinduction ua haujlwm li cas?
Lub hauv paus ntsiab lus ntawm autoinduction media yog raws li cov pa roj carbon monoxide hauv nruab nrab uas yog metabolized sib txawv los txhawb kev loj hlob ntawm tes loj thiab tau txais kev txhawb nqa cov protein uas tau tsav los ntawm cov neeg txhawb nqa lac.
Dab tsi yog autoinduction hauv protein qhia?
Autoinduction yog ib txoj hauv kev yooj yim rau kev qhia cov protein uas xav tau kev cuam tshuam cov neeg siv me me tom qab inoculation ntawm kab lis kev cai (Studier, 2005).
Dab tsi yog qhov nruab nrab Autoinduction?
Auto Induction Media (AIM) tau tsim los kom loj hlob IPTG-inducible qhia hom, pib yam tsis muaj induction, thiab tom qab ntawd ua rau tsim cov phiaj protein tau txais txiaj ntsig, feem ntau ze ze ntawm saturation ntawm siab ceev ceev.
Koj siv Autoinduction media li cas?
Siv rau T7 thiab pTrc
Glucose, glycerol, thiab lactose tau ntxiv rau cov poov xab bufferedua autoinduction media. Raws li E. coli kab lis kev cai loj hlob, lawv noj cov piam thaj ua ntej. Raws li cov piam thaj dhau lawm, lawv raug yuam kom siv cov lactose uas ua rau kev qhia ntawm T7 txhawb nqa.
