Arabinose yog tsib-carbon qab zib uas pom dav hauv cov xwm txheej thiab tuaj yeem ua cov pa roj carbon monoxide hauv ntau cov kab mob. Cov khoom lag luam protein los ntawm peb lub noob (araB, araA, thiab araD) yog xav tau rau arabinose degradation hauv cov tswv cuab ntawm tsev neeg Enterobacteriaceae, xws li E. coli thiab S.
Arabinose ua dab tsi rau cov kab mob?
Yog li thaum arabinose tshwm sim los qhib lub arabinose operon, GFP yog tsim tawm thiab cov kab mob tuaj yeem fluoresce. Yog tsis muaj arabinose, GFP noob tsis qhia thiab tsis muaj fluorescence. Yog li los ntawm kev sib txawv ntawm cov khoom noj muaj pes tsawg leeg, tus kws tshawb fawb tuaj yeem tswj tau thaum twg (thiab yog tias) florescence tshwm sim hauv cov kab mob hloov pauv.
Lub luag haujlwm ntawm arabinose yog dab tsi hauv kev hloov cov kab mob?
Arabinose ua ua tus tswj hwm allosteric ntawm AraC, hloov pauv DNA qhov chaw nws khi rau thiab ua li cas nws tsim lub dimer. Nco ntsoov tias arabinose yog cov suab thaj uas tau txais catabolized los ntawm cov proteins ntawm AraBAD operon. Thaum arabinose ntxiv rau ib puag ncig uas E. coli nyob, nws khi nruj rau AraC.
Vim li cas arabinose siv?
Siv hauv biology
Nyob rau hauv hluavtaws biology, arabinose feem ntau yog siv raws li a ib-txoj kev los yog thim rov qab mus rau cov protein qhia nyob rau hauv Pphem txhawb nqa hauv E. coli. Qhov kev hloov pauv no tuaj yeem cuam tshuam los ntawm kev muaj cov piam thaj los yog thim rov qab los ntawm kev ntxiv cov piam thaj hauv kab lis kev cai nruab nrab uas yog ib hom kev tawm tsam catabolite.
HowPuas yog arabinose cuam tshuam rau kev tswj cov noob thiab kev qhia hauv cov kab mob?
Thaum arabinose tam sim no, araC so cov DNA teeb tsa kom qhov kev hloov pauv tuaj yeem tshwm sim, tab sis thaum tsis muaj cov carbohydrates no, tib cov protein txwv cov DNA kom txo nws lub peev xwm los khi Nrog RNA polymerase.
