Proteins yog denatured los ntawm kev kho mob alkaline lossis acid, oxidizing lossis txo cov neeg ua haujlwm, thiab qee cov organic solvents. Txaus siab ntawm cov neeg ua haujlwm denaturing yog cov uas cuam tshuam rau cov qauv theem nrab thiab theem nrab yam tsis muaj kev cuam tshuam rau lub hauv paus qauv.
Yuav ua li cas 3 yam uas tuaj yeem ua rau cov proteins?
Kub, pH, salinity, polarity ntawm cov kuab tshuaj - cov no yog qee yam uas cuam tshuam rau cov duab ntawm cov protein. Yog tias ib qho lossis kev sib xyaw ua ke ntawm cov xwm txheej no txawv ntawm cov xwm txheej ib txwm ua, cov duab (thiab kev ua haujlwm) ntawm cov protein yuav hloov. Qhov kev hloov pauv no tseem hu ua denatured.
Dab tsi tuaj yeem pom cov piv txwv protein?
Cov piv txwv ntau
Thaum zaub mov siav, qee qhov ntawm nws cov proteins dhau los. Qhov no yog vim li cas cov qe boiled ua tawv thiab cov nqaij siav yuav khov. Ib qho piv txwv classic ntawm denaturing hauv cov protein yog los ntawm qe dawb, uas yog qe albumins loj hauv dej.
YYuav ua li cas thiaj ua tau cov protein denature?
Denaturation koom nrog kev tawg ntawm ntau qhov kev sib txuas tsis muaj zog, lossis kev sib cog lus (piv txwv li, hydrogen bonds), nyob rau hauv ib qho protein molecule uas yog lub luag haujlwm rau cov qauv ntawm cov protein ntau. nyob rau hauv nws natural (native) xeev. Denatured proteins muaj ib tug looser, ntau random qauv; feem ntau yog insoluble.
Yuav ua li cas thiaj hais tau cov lus nug protein?
Cov ntsiab lus hauv cov teeb no (6)
- Denaturation. hais txog cov kev hloov ntawm lub cev uas coj musqhov chaw nyob rau hauv cov protein raug rau qhov txawv txav nyob rau hauv ib puag ncig.
- Kub / Kub. cuam tshuam H-bonds thiab hydrophobic kev sib cuam tshuam ntawm cov tshuaj tiv thaiv tsis-polar. …
- Acid/Bases. …
- YOrganic Compounds. …
- Heavy Hlau Ions. …
- Kev ntxhov siab.
