Proteins yog denatured los ntawm kev kho mob alkaline lossis acid, oxidizing lossis txo cov neeg ua haujlwm, thiab qee cov organic solvents. Txaus siab ntawm cov neeg ua haujlwm denaturing yog cov uas cuam tshuam rau cov qauv theem nrab thiab theem nrab yam tsis muaj kev cuam tshuam rau lub hauv paus qauv.
Cov proteins puas tuaj yeem ua kom sov lossis kua qaub?
Ib qho protein ua denatured thaum nws cov duab ib txwm tau deformedvim tias qee qhov ntawm hydrogen bonds tawg. Tsis muaj zog hydrogen bonds tawg thaum tshav kub heev los yog thaum lawv raug rau cov kua qaub (xws li citric acid los ntawm txiv qaub kua txiv).
Yuav ua li cas 3 yam tuaj yeem tsim cov proteins?
Kub, pH, salinity, polarity ntawm cov kuab tshuaj - cov no yog qee yam uas cuam tshuam rau cov duab ntawm cov protein. Yog tias ib qho lossis kev sib xyaw ua ke ntawm cov xwm txheej no txawv ntawm cov xwm txheej ib txwm ua, cov duab (thiab kev ua haujlwm) ntawm cov protein yuav hloov. Qhov kev hloov pauv no tseem hu ua denatured.
YYuav ua li cas thiaj muaj protein ntau?
Siv cua sov. Thaum tshav kub kub yog ib txoj hauv kev yooj yim tshaj plaws thiab feem ntau txoj hauv kev los denature protein. Thaum cov protein nyob rau hauv nqe lus nug nyob rau hauv cov zaub mov, tsuas yog ua noj cov zaub mov yuav denature cov proteins. Ntau cov proteins tuaj yeem ua kom pom tseeb los ntawm kev nthuav tawm lawv mus rau qhov kub ntawm lossis siab dua 100 ° C (212 ° F).
Dab tsi ua rau muaj protein ntau?
Yog tias cov protein poob nws cov duab, nws yuav tsum tsis ua haujlwm. Cov txheej txheem uas ua rau cov protein poob nws cov duab yoglub npe hu ua denaturation. Denaturation feem ntau yog tshwm sim los ntawm kev ntxhov siab sab nraud ntawm cov protein, xws li cov kuab tshuaj, cov ntsev inorganic, raug rau cov kua qaub lossis cov hauv paus, thiab los ntawm tshav kub.
