Nws yog ib qho tseem ceeb heev uas yuav tsum paub tias cov ntaub ntawv tsim tawm nrog rau sab hnub poob blot feem ntau suav hais tias yog ib feem ntau. Qhov no yog vim nws muab kev sib piv ntawm cov protein ntau, tab sis tsis yog qhov ntsuas qhov muaj tseeb.
Puas yog western blot qualitative or quantitative?
Western blot yog txhim khu kev qha ntau txoj kevtsuas yog yog cov qauv khoom thiab kev ncaj ncees, cov tshuaj tiv thaiv kab mob rau lub hom phiaj protein, thiab cov txheej txheem thauj khoom raug txiav txim siab. Ua tib zoo saib xyuas cov ntsiab lus, koj tuaj yeem zam qhov yuam kev ntau thiab zam kev txhais cov ntaub ntawv Western blot tsis raug.
Yuav ua li cas hom kev western blot?
Ab western blot yog ib txoj kev sim siv los kuaj xyuas cov protein molecules los ntawm cov sib xyaw ntawm cov proteins. Qhov sib xyaw no tuaj yeem suav nrog tag nrho cov proteins uas cuam tshuam nrog cov ntaub so ntswg lossis hom cell.
Pab western blot quantify?
Western blots tau kuaj pom nrog tshuaj tiv thaiv tshwj xeeb rau lub hom phiaj protein uas paub tias yog thawj cov tshuaj tiv thaiv. … Txawm li cas los xij, nws tseem tuaj yeem siv Western blotting los muab qhov tseeb ntawm cov proteins hauv cov qauv, txhawm rau ntsuas kev hloov pauv hauv cov qib qhia protein (2).
Puas western blot kom muaj nuj nqis proteins?
Tom qab lysis ntawm cov hlwb, nws yog ib qho tseem ceeb rau txiav txim siab tag nrho cov protein ntau ntawm cov qauv. Qhov tseeb kom muaj nuj nqis ntawm cov qauv yuav tso cai rau koj thauj khoomCov protein ntau npaum li cas hauv txhua txoj kab.
