2024 Tus sau: Elizabeth Oswald | [email protected]. Kawg hloov kho: 2024-01-13 00:07
Densitometry cov ntaub ntawv tsim tawm rau Western blots yog nquag siv los sib piv cov protein ntau ntawm cov qauv. … Cov ntaub ntawv nonlinear densitometry tau pom thaum Western blots tau kuaj pom siv infrared fluorescence lossis chemiluminescence, thiab nyob rau hauv cov xwm txheej SDS-PAGE sib txawv.
Densitometry tsom xam yog dab tsi?
Densitometry yog qhov ntsuas qhov ntsuas qhov muag ntawm qhov muag pom hauv cov khoom siv lub teeb pom kev zoo, xws li cov ntawv thaij duab lossis cov duab thaij duab, vim raug lub teeb.
Koj siv densitometry li cas hauv duab J?
Densitometry siv ImageJ
- Nias qhov nruab nrab ntawm lub xwmfab thiab rub nws hla mus rau txoj kab tom ntej. …
- Rau txoj kab kawg, rov ua cov txheej txheem tab sis nias Ctrl thiab 3 los teeb tsa txoj kab kawg. …
- Siv cov cuab yeej los kos cov kab kom tshem tawm txoj kab tom qab los ntawm kev suav. …
- Mus rau: Kev Ntsuam Xyuas → Gels → Label Peaks kom tau txais tsab ntawv ceeb toom.
Yuav ua li cas koj txheeb xyuas cov txiaj ntsig western blot?
Txheeb xyuas koj cov cuab yeej kom muaj nuj nqis . Piv txwv li, khiav ib txoj kab uas muaj 20 µg, 15 µg, thiab 10 µg ntawm tag nrho cov protein. Tom qab kev txheeb xyuas, qhov txheeb ze ntawm cov phiaj bands yuav tsum yog 2, 1.5, thiab 1. Sim sib txawv kom muaj nuj nqis cov cuab yeej thiab teeb tsa kom txog thaum koj tuaj yeem txhim kho cov txiaj ntsig raug.
Yuav ua li cas koj thiaj suav tau ib lub tebchaws sab hnub poob?
Kauj Ruam 1: Txiav txim siab qhov keeb kwm yav dhau los rho tawmQhov ceev ntawm koj cov protein ntawm kev txaus siab (PI) thiab normalizing tswj (NC). Kauj Ruam 2: Txheeb xyuas NC uas muaj tus nqi siab tshaj plaws. Kauj Ruam 3: Faib tag nrho cov txiaj ntsig NC los ntawm qhov siab tshaj plaws NC tus nqi kom tau tus nqi NC txheeb ze.
Pom zoo:
Puas yog cov dab dab dab dab dab dab dab dab dab dab dab dab?
Myth: Ib tug dab tshos tsis yog neeg ncaj ncees yog tiasnws haum yog. Fact: Tu siab, qhov no yog ib lo lus tsis tseeb uas tau raug perpetuated los ntawm aversive trainers. Txawm hais tias haum rau lub caj dab kom zoo khawb rau hauv cov tawv nqaij rhiab nyob ib ncig ntawm lub caj dab, uas ua rau muaj kev puas tsuaj loj rau cov thyroid, esophagus, thiab trachea.
Dab tsi yog kev tswj kev thauj khoom nyob rau sab hnub poob blot?
Kev tswj cov tshuaj tiv thaiv kab mob yog qhov tseem ceeb ntawm kev tswj hwm raws li lawv qhia qhov sib npaug ntawm cov qauv thauj khoom thoob plaws txhua qhov dej. Chaw thau khoom tswj kuj tseem qhia qhov kev hloov pauv ntawm cov protein mus rau daim nyias nyias thaum lub sij hawm western blotting txheej txheem.
Vim li cas western blot semi quantitative?
Nws yog ib qho tseem ceeb heev uas yuav tsum paub tias cov ntaub ntawv tsim tawm nrog rau sab hnub poob blot feem ntau suav hais tias yog ib feem ntau. Qhov no yog vim nws muab kev sib piv ntawm cov protein ntau, tab sis tsis yog qhov ntsuas qhov muaj tseeb.
Dab tsi yog tus bestiary hauv dab dab dab?
Wiki Targeted (Kev Ua Si) Beasts yog cov lus hu uas muab Metropolitan Era monsters txhawb nqa rau stats nrog rau cov txiaj ntsig hauv kev sib ntaus sib tua. Tus Bestiary tuaj yeem kho tau ntawm qib 18, qhov twg cov neeg ua si tuaj yeem qhib thiab nce qib Beasts los txhim kho lawv lub zog thiab cov teebmeem.
Puas yog cov dab dab dab dab dab dab tau them?
OHL cov neeg ua si tsis tau nyiaj hli, vim lawv tsis raug lees paub tias yog cov neeg ua haujlwm raws li Txoj Cai Kev Ua Haujlwm. Txawm li cas los xij, lawv suav nrog cov txiaj ntsig xws li nyiaj rov qab txhua xyoo, tag nrho cov nqi kawm ntawv them, thiab daim pib pub dawb.
