Kev tswj cov tshuaj tiv thaiv kab mob yog qhov tseem ceeb ntawm kev tswj hwm raws li lawv qhia qhov sib npaug ntawm cov qauv thauj khoom thoob plaws txhua qhov dej. Chaw thau khoom tswj kuj tseem qhia qhov kev hloov pauv ntawm cov protein mus rau daim nyias nyias thaum lub sij hawm western blotting txheej txheem. Loading controls feem ntau yog cov proteins uas muaj siab thiab nthuav dav.
Kev tswj kev thauj khoom ua li cas?
Kev tswj kev thauj khoom yog cov protein siv los tswj hauv Western blotting sim. … Lawv yog siv kom paub tseeb tias cov protein tau thauj khoom sib npaug thoob plaws txhua qhov dej.
Vim li cas peb thiaj li siv kev tswj hwm kev thauj khoom hauv Western blot?
Kev tswj hwm kev thauj khoom muaj lub luag haujlwm thib ob raws li kev tswj hwm sab hnub poob blots. Lawv tuaj yeem siv txhawm rau txheeb xyuas tias txawm tias tau hloov pauv los ntawm cov gel mus rau daim nyias nyias hla tag nrho cov gel. Qhov no yog qhov tseem ceeb thaum kev sib piv yog ua los ntawm cov protein qhia qib ntawm cov qauv.
Koj siv qhov kev tswj hwm kev thauj khoom hauv Western blot li cas?
Cov teeb liab los ntawm kev tswj hwm kev thauj khoom feem ntau yog siv kom normalize cov teeb liab los ntawm cov proteins txaus siab. Txhawm rau siv kev tswj hwm kev thauj khoom rau cov laj thawj no, kev tshawb nrhiav nrog kev tswj hwm cov tshuaj tiv thaiv kab mob thiab cov tshuaj tiv thaiv kev sim yuav tsum tau ua ntawm tib lub blot. Ntau hom proteins sib txawv yog siv los tswj kev thauj khoom.
Vim li cas actin thiaj siv los tswj kev thauj khoom?
Beta-actin, feem ntau yog siv los tswj kev thauj khoom rau Western Blot raunormalize cov qib protein uas kuaj pom los ntawm kev lees paub tias kev thauj khoom protein yog tib yam thoob plaws gel.
