2024 Tus sau: Elizabeth Oswald | [email protected]. Kawg hloov kho: 2024-01-13 00:07
Kev tswj cov tshuaj tiv thaiv kab mob yog qhov tseem ceeb ntawm kev tswj hwm raws li lawv qhia qhov sib npaug ntawm cov qauv thauj khoom thoob plaws txhua qhov dej. Chaw thau khoom tswj kuj tseem qhia qhov kev hloov pauv ntawm cov protein mus rau daim nyias nyias thaum lub sij hawm western blotting txheej txheem. Loading controls feem ntau yog cov proteins uas muaj siab thiab nthuav dav.
Kev tswj kev thauj khoom ua li cas?
Kev tswj kev thauj khoom yog cov protein siv los tswj hauv Western blotting sim. … Lawv yog siv kom paub tseeb tias cov protein tau thauj khoom sib npaug thoob plaws txhua qhov dej.
Vim li cas peb thiaj li siv kev tswj hwm kev thauj khoom hauv Western blot?
Kev tswj hwm kev thauj khoom muaj lub luag haujlwm thib ob raws li kev tswj hwm sab hnub poob blots. Lawv tuaj yeem siv txhawm rau txheeb xyuas tias txawm tias tau hloov pauv los ntawm cov gel mus rau daim nyias nyias hla tag nrho cov gel. Qhov no yog qhov tseem ceeb thaum kev sib piv yog ua los ntawm cov protein qhia qib ntawm cov qauv.
Koj siv qhov kev tswj hwm kev thauj khoom hauv Western blot li cas?
Cov teeb liab los ntawm kev tswj hwm kev thauj khoom feem ntau yog siv kom normalize cov teeb liab los ntawm cov proteins txaus siab. Txhawm rau siv kev tswj hwm kev thauj khoom rau cov laj thawj no, kev tshawb nrhiav nrog kev tswj hwm cov tshuaj tiv thaiv kab mob thiab cov tshuaj tiv thaiv kev sim yuav tsum tau ua ntawm tib lub blot. Ntau hom proteins sib txawv yog siv los tswj kev thauj khoom.
Vim li cas actin thiaj siv los tswj kev thauj khoom?
Beta-actin, feem ntau yog siv los tswj kev thauj khoom rau Western Blot raunormalize cov qib protein uas kuaj pom los ntawm kev lees paub tias kev thauj khoom protein yog tib yam thoob plaws gel.
Pom zoo:
Puas yog cov dab dab dab dab dab dab dab dab dab dab dab dab?
Myth: Ib tug dab tshos tsis yog neeg ncaj ncees yog tiasnws haum yog. Fact: Tu siab, qhov no yog ib lo lus tsis tseeb uas tau raug perpetuated los ntawm aversive trainers. Txawm hais tias haum rau lub caj dab kom zoo khawb rau hauv cov tawv nqaij rhiab nyob ib ncig ntawm lub caj dab, uas ua rau muaj kev puas tsuaj loj rau cov thyroid, esophagus, thiab trachea.
Leej twg sab qab teb sab hnub tuaj yog thawj zaug uas tau tsiv mus rau sab hnub poob?
Choctaw yog thawj qhov kev sib tham ua tiav: xyoo 1830 lawv tau pom zoo muab lawv cov cuab yeej cuab tam rau thaj av sab hnub poob, kev thauj mus los rau lawv tus kheej thiab lawv cov khoom, thiab kev txhawb nqa thaum lub sijhawm thiab tom qab taug kev.
Puas yog basingstoke sab qab teb sab hnub tuaj lossis sab hnub poob?
Basingstoke nyob hauv lub nroog Hampshire, South East England, xya mais sab qab teb-sab hnub tuaj ntawm lub nroog Tadley, thiab 45 mais sab qab teb-sab hnub poob ntawm London. Basingstoke nyob rau mais sab qab teb ntawm Berkshire ciam teb. cheeb tsam twg yog Basingstoke hauv?
Vim li cas ho tsis ya mus rau sab hnub poob mus rau sab hnub poob tseem ceeb?
Southwest Airlines tau hais rau hnub Thursday nws yuav tsum tsis ua haujlwm hauv peb lub nroog, suav nrog Key West. "Hmoov tsis zoo,theem ntawm kev thov hauv zos tsis tso cai rau Southwest kom tau txais txiaj ntsig zoo rau cov lag luam no,"
Zoo sab hnub poob yuav ya mus rau sab hnub poob tseem ceeb?
Southwest Airlines tam sim no tsis muaj kev ya mus rau Key West, Florida. Vim li cas Southwest nres ya mus rau Key West? Southwest Airlines tau hais rau hnub Thursday nws yuav tsum tsis ua haujlwm hauv peb lub nroog, suav nrog Key West.
