Pipet cov nqaij zom cov nqaij pellet nce thiab nqis rau 20-30 zaug siv lub sterile 10 ml pipette. Qhov no ua kom ntseeg tau tias feem ntau myoblasts raug tso tawm los ntawm cov leeg nqaij. Muab ib tug 70 μm lim rau ntawm 50 ml raj. Ua ntej ntub dej ntawm 70 μm lim nrog 2 ml ntawm qhov nruab nrab / cais tawm tawm.
Dab tsi yog qhov tseem ceeb myoblasts?
Primary myoblasts yog undifferentiated proliferating precursors skeletal nqaij. Lawv tuaj yeem raug coj los ua thiab kawm ua cov leeg nqaij ua ntej lossis txhawb kom sib txawv rau cov theem tom qab ntawm cov leeg nqaij.
myoblasts txawv ntawm myotubes li cas?
MyoD thiab Myf5 ua kom muaj kev sib txawv ntawm myogenic progenitors rau hauv myoblasts, ua raws li myogenin, uas sib txawv cov myoblast rau hauv myotubes. MRF4 yog ib qho tseem ceeb rau kev thaiv cov kev hloov pauv ntawm cov leeg nqaij tshwj xeeb, ua rau cov nqaij pob txha progenitors loj hlob thiab loj hlob ua ntej sib txawv.
Dab tsi yog myoblasts?
: ib qho tsis sib xws ntawm tes muaj peev xwm ua kom nce mus rau cov leeg nqaij.
C2C12 cell kab yog dab tsi?
C2C12 xovtooj ntawm tes yog a subclone ntawm myoblasts uas yog thawj zaug tau los ntawm Yaffe thiab Saxel ntawm Weizmann Institute of Science hauv Ixayees xyoo 1977. … Cov hlwb no muaj peev xwm ceev ceev. Kev loj hlob nyob rau hauv cov ntshav cov ntshav siab thiab sib txawv rau hauv myotubes nyob rau hauv cov ntshav qis.
