Kev hloov pauv hloov pauv hloov pauv (TMA) yog qhov isothermal (tsis hloov pauv nucleic acid kub), ib leeg-tube nucleic acid amplification system siv ob lub enzymes, RNA polymerase thiab thim rov qab kev sau ntawv. … Cov txheej txheem no tuaj yeem siv los tsom rau RNA thiab DNA.
TMA molecular yog dab tsi?
Ib qho Isothermal Molecular Amplification txheej txheem
Kev Tshaj Tawm-Mediated Amplification (TMA) yog ib qho RNA transcription-mediated amplification system siv ob lub enzymes los tsav cov tshuaj tiv thaiv: RNA polymerase thiab thim rov qab transcriptase.
Strand displacement amplification ua haujlwm li cas?
Strand Displacement Amplification (SDA) yog ib qho isothermal, hauv vitro nucleic acid amplification txheej txheem raws li lub peev xwm ntawm HincII rau nick lub unmodified strand ntawm ib hemiphosphorothioate daim ntawv ntawm nws qhov chaw paub, thiab muaj peev xwm ntawm exonuclease deficient klenow (exo- klenow) mus txuas ntxiv 3'-kawg ntawm lub npe…
Yuav ua li cas recombinase polymerase amplification ua haujlwm?
Recombinase polymerase amplification (RPA) yog ib lub raj, isothermal lwm txoj rau polymerase saw cov tshuaj tiv thaiv (PCR). Los ntawm ntxiv qhov thim rov qab transcriptase enzyme rau RPA cov tshuaj tiv thaiv nws tuaj yeem ntes RNA nrog rau DNA, tsis tas yuav tsum muaj ib kauj ruam cais los tsim cDNA,.
Lub luag haujlwm ntawm recombinase yog dab tsi?
Recombinases yog enzymes uas catalyze qhov chaw tshwj xeeb recombination txheej xwm hauv DNA; rauPiv txwv li, genetic recombination thaum lub sij hawm meiosis nyob rau hauv uas recombination ua hauj lwm los tsim kev sib txuas tshiab ntawm alleles ntawm chromosomes. … Recombinases kuj ua haujlwm hauv kev kho DNA recombinational.
