2024 Tus sau: Elizabeth Oswald | [email protected]. Kawg hloov kho: 2024-01-13 00:07
Kev hloov pauv hloov pauv hloov pauv (TMA) yog qhov isothermal (tsis hloov pauv nucleic acid kub), ib leeg-tube nucleic acid amplification system siv ob lub enzymes, RNA polymerase thiab thim rov qab kev sau ntawv. … Cov txheej txheem no tuaj yeem siv los tsom rau RNA thiab DNA.
TMA molecular yog dab tsi?
Ib qho Isothermal Molecular Amplification txheej txheem
Kev Tshaj Tawm-Mediated Amplification (TMA) yog ib qho RNA transcription-mediated amplification system siv ob lub enzymes los tsav cov tshuaj tiv thaiv: RNA polymerase thiab thim rov qab transcriptase.
Strand displacement amplification ua haujlwm li cas?
Strand Displacement Amplification (SDA) yog ib qho isothermal, hauv vitro nucleic acid amplification txheej txheem raws li lub peev xwm ntawm HincII rau nick lub unmodified strand ntawm ib hemiphosphorothioate daim ntawv ntawm nws qhov chaw paub, thiab muaj peev xwm ntawm exonuclease deficient klenow (exo- klenow) mus txuas ntxiv 3'-kawg ntawm lub npe…
Yuav ua li cas recombinase polymerase amplification ua haujlwm?
Recombinase polymerase amplification (RPA) yog ib lub raj, isothermal lwm txoj rau polymerase saw cov tshuaj tiv thaiv (PCR). Los ntawm ntxiv qhov thim rov qab transcriptase enzyme rau RPA cov tshuaj tiv thaiv nws tuaj yeem ntes RNA nrog rau DNA, tsis tas yuav tsum muaj ib kauj ruam cais los tsim cDNA,.
Lub luag haujlwm ntawm recombinase yog dab tsi?
Recombinases yog enzymes uas catalyze qhov chaw tshwj xeeb recombination txheej xwm hauv DNA; rauPiv txwv li, genetic recombination thaum lub sij hawm meiosis nyob rau hauv uas recombination ua hauj lwm los tsim kev sib txuas tshiab ntawm alleles ntawm chromosomes. … Recombinases kuj ua haujlwm hauv kev kho DNA recombinational.
Pom zoo:
Ntawm kev pauv pauv pauv hloov pauv?
Kev sib pauv pauv hloov pauv yog daim ntawv cog lus nyiaj txiag uas tau teev tseg thiab ua lag luam ntawm kev sib pauv hloov pauv. Qhov yooj yim hais, cov no yog cov khoom siv uas tau pauv pauv hauv kev tswj hwm. … Cov yav tom ntej thiab cov kev xaiv yog ob qho kev sib pauv uas nrov tshaj plaws.
Qhov kev hloov pauv no puas yuav ua rau muaj kev hloov pauv hloov pauv?
Yog li, yog tias muaj kev hloov pauv, piv txwv li, kev ntxig lossis tshem tawm ntawm nucleotide, tshwm sim, qhov no tuaj yeem ua rau kev hloov pauv ntawm kev nyeem ntawv. Nws hloov tag nrho cov amino acid ib ntus. Cov kev hloov pauv no yog hu ua frameshift mutation (tseem hu ua kev nyeem ntawv hloov pauv, nyeem ntawv hloov pauv, lossis framing yuam kev).
Qhov twg yog qhov kev hloov pauv hloov pauv hloov pauv?
Semiconservative replication piav qhia txog cov txheej txheem ntawm DNA replication nyob rau hauv tag nrho cov paub hlwb. DNA replication tshwm sim ntawm ntau lub hauv paus pib ntawm kev rov ua dua raws DNA qauv strand. Raws li DNA ob chav helix yog unwound by helicase, replication tshwm sim nyias ntawm txhua tus qauv strand nyob rau hauv antiparallel cov lus qhia.
Qhov kev hloov pauv twg yog xav tias yuav cuam tshuam los ntawm kev hloov pauv hloov pauv?
Dependent Variable Qhov sib txawv uas nyob ntawm lwm yam uas ntsuas. Cov kev hloov pauv no yuav tsum tau hloov pauv raws li qhov kev sim ua haujlwm ntawm tus kheej hloov pauv lossis hloov pauv. Nws yog cov nyhuv presumed. Qhov kev tshawb fawb hloov pauv twg yog qhov tshwm sim?
Kev hloov pauv dav dav txawv li cas los ntawm kev hloov pauv tshwj xeeb?
Muaj ob hom kev hloov pauv: dav dav thiab tshwj xeeb. Hauv kev hloov pauv dav dav, tus kab mob bacteriophages tuaj yeem khaws ib feem ntawm tus tswv tsev genome. Hauv qhov sib piv, nrog kev hloov pauv tshwj xeeb, cov kab mob bacteriophages khaws tsuas yog qee qhov tshwj xeeb ntawm tus tswv tsev DNA.
