Vim tias cov phab ntsa ntawm tes tiv taus ntau cov tebchaw, cov kab mob acid-ceev ceev yuav tsum muaj cov txheej txheem tshwj xeeb staining. Thawj cov stain siv hauv acid-ceev staining, carbolfuchsin, yog lipid-soluble thiab muaj phenol, uas pab cov stain nkag mus rau ntawm phab ntsa ntawm tes. Qhov no yog pab ntxiv los ntawm ntxiv rau cua sov.
Vim li cas thiaj yuav tsum tau cua sov rau cov kua qaub-ceev staining?
Cua sov lub swb pab kom muag cov khoom siv quav ciab ntawm cov kab mob ntawm phab ntsa. Cov khoom siv waxy yog hydrophobic rau aqueous tov tab sis tsis yog rau phenolic daws ntawm yooj yim fuchsin. Yog li muaj zog carbol fuchsin muaj peev xwm stain lub cell. Thaum staining, lawv zoo li tiv thaiv decolourization los ntawm 20% H2SO4 (sulphuric acid).
Yuav ua li cas koj cov kua qaub nrawm?
Acid-Fast Staining Instructions
- Cua qhuav thiab cua sov kho cov yeeb yaj kiab nyias ntawm cov kab mob. …
- Dej nyab lub swb nrog Carbolfuchsin. …
- Dej swb nrog Acid Cawv rau 30 vib nas this. …
- Counterstain los ntawm dej nyab lub swb nrog Methylene Blue rau 30 vib nas this. …
- Kua tus swb los ntawm muab tso rau nruab nrab ntawm nplooj ntawv ntawm phau ntawv Bibulous.
Puas Gram staining xav tau cua sov?
A Gram stain feem ntau yog ua los ntawm kev npaj smear uas tau kho cua sov. Ib txoj haujlwm ntawm fixation yog kev ruaj ntseg (kho) cov hlwb rau cov swb. Hauv biofilm, txawm li cas los xij, cov hlwb twb txuas nrog. Tsis tas li ntawd, lub tshav kub khoswb yog qhuav, tab sis ib tug biofilm feem ntau yog dej.
Dab tsi stain tsis xav tau cua sov?
Nws suav nrog daim ntawv thov ntawm cov stains (basic fuchsin), decolorizer (acid-alcohol), thiab counterstain (methylene xiav). Tsis zoo li Ziehl–Neelsen stain (Z-N stain), txoj kev Kinyoun ntawm staining tsis tas yuav cua sov.
