Nyob hauv physics, qhov teeb meem theem yog qhov teeb meem ntawm cov ntaub ntawv poob txog theem uas tuaj yeem tshwm sim thaum ntsuas lub cev. Lub npe los ntawm daim teb ntawm X-ray crystallography, qhov twg cov theem teeb meem yuav tsum tau daws rau kev txiav txim ntawm ib tug qauv ntawm cov ntaub ntawv diffraction.
Yuav ua li cas cov teeb meem theem daws rau cov proteins?
Qhov teeb meem feem ntau tuaj yeem daws tau los ntawm siv hnyav atom derivatized crystals (saib piv txwv li, Watenpaugh, 1985). Rau txhua pawg ntawm Bragg indices hkl cov qauv ntawm cov haiv neeg daim ntawv Fp yog muab piv nrog uas los ntawm ib tug hnyav atom derivative siv lead ua Fph.
Kev txiav txim theem yog dab tsi?
Txhua qhov kev xav ntawm tus qauv diffraction lossis qauv qauv sib raug rau nthwv dej uas muaj amplitude thiab theem. … Lub amplitude tau yooj yim xam los ntawm kev noj cov hauv paus square ntawm qhov kev siv, tab sis cov theem poob thaum lub sij hawm sau cov ntaub ntawv.
Vim li cas cov ntaub ntawv theem poob?
Cov teeb meem theem tshwm sim vim nws tsuas yog ua tau los ntsuas qhov amplitude ntawm diffraction me ntsis: cov ntaub ntawv ntawm cov theem ntawm cov hluav taws xob diffraction ploj lawm. Cov txheej txheem muaj los tsim kho cov ntaub ntawv no.
Dab tsi yog qhov txwv ntawm X-ray crystallography?
Qhov tsis zoo ntawm X-ray crystallography suav nrog: Cov qauv yuav tsum yog crystallizable . Cov homcov qauv uas tuaj yeem txheeb xyuas tau txwv. Tshwj xeeb tshaj yog, daim nyias nyias cov proteins thiab cov molecules loj yog qhov nyuaj rau crystallize, vim lawv qhov hnyav molecular thiab cov solubility tsis zoo.
