Disulfide daim ntawv cog lus koom nrog cov tshuaj tiv thaiv ntawm sulfhydryl (SH) sab chains ntawm ob cysteine residues: ib qho S- anion los ntawm ib pawg sulfhydryl ua raws li ib tug nucleophile, tawm tsam sab ntawm cov cysteine thib ob los tsim ib daim ntawv cog lus disulfide, thiab hauv cov txheej txheem tso tawm electrons (txo qhov sib npaug) hloov mus.
Disulfide txuas hniav nyob qhov twg?
Disulfide daim ntawv cog lus feem ntau tshwm sim hauv lub endoplasmic reticulum los ntawm oxidation. Yog li no disulfide bonds feem ntau pom muaj nyob rau hauv extracellular, secreted thiab periplasmic proteins, txawm hais tias lawv muaj peev xwm kuj tsim nyob rau hauv cytoplasmic proteins nyob rau hauv tej yam kev mob ntawm oxidative kev nyuaj siab.
Yuav ua li cas disulfide bonds tsim nyob rau hauv lub cell?
Protein disulphide bonds yog tsim nyob rau hauv endoplasmic reticulum ntawm eukaryotic hlwb thiab periplasmic chaw ntawm cov hlwb prokaryotic. Txoj hauv kev tseem ceeb uas ua rau muaj kev tsim cov protein disulphide daim ntawv cog lus hauv prokaryotes thiab eukaryotes yog qhov zoo sib xws, thiab lawv muaj ntau yam kev siv tshuab.
Dab tsi yog disulfide daim ntawv cog lus?
Kev tsim ntawm cov nyiaj disulfide (DSBs) hauv cov protein yog cov txheej txheem oxidative uas tsim kom muaj covalent daim ntawv cog lus txuas cov sulfur atoms ntawm ob cysteine residues. DSBs pab txhawb kev ua haujlwm ntawm ntau cov proteins los ntawm kev ruaj khov rau hauv lawv cov kev sib raug zoo.
Yuav ua li cas daim ntawv cog lus ua disulfide txuas rau qhov twglawv tsim?
Ib daim ntawv cog lus disulfide yog covalent daim ntawv cog lus ntawm ob lub sulfur atoms (–S–S–) tsim los ntawm kev sib txuas ntawm ob pawg thiol (–SH). Cysteine, ib qho ntawm 20 protein amino acids, muaj ib pab pawg -SH nyob rau hauv nws sab saw, thiab tau yooj yim yuav dimereized rau cystine nyob rau hauv aqueous tov los ntawm tsim ib tug disulfide daim ntawv cog lus.
