Txoj Cai. Qhov no yog cov tshuaj tiv thaiv dav dav rau pentose thiab nyob ntawm qhov tsim ntawm furfural. Thaum pentose yog rhuab nrog concentration HCl, orcinol reacts nyob rau hauv lub xub ntiag ntawm furfural nyob rau hauv lub xub ntiag ntawm ferric chloride raws li ib tug catalyst purine los tsim ntsuab xim tsuas yog purine nucleotide.
siv orcinol yog dab tsi?
A txheej txheem siv los kuaj pom qhov muaj pentoses nrogib qho tshuaj reagent uas muaj orcinol, HCl thiab ferric chloride. Qhov kev sim no yog siv los kuaj pom qhov muaj pentose hauv cov zis. Nyob rau hauv lub xub ntiag ntawm pentoses, qhov kuaj reagent dehydrates pentoses los ua furfural.
tshuaj tiv thaiv orcinol yog dab tsi?
Cov orcinol reagent reacts nrog pentosepawg hauv qaum qaum ntawm RNA molecule thiab nyob ntawm qhov tsim ntawm furfural, thaum pentose yog rhuab nrog concentrated hydrochloric acid. Orcinol reacts nrog furfural nyob rau hauv lub xub ntiag ntawm ferric chloride ua ib tug catalyst muab ib tug ntsuab xim.
orcinol pab li cas hauv kev kwv yees RNA?
Kev Taw Qhia: HiPer® RNA Kev Ntsuas Kev Qhia Cov Khoom Siv yog tsim los rau kev txiav txim siab sai thiab raug ntawm RNA los ntawm orcinol reagent. Txoj kev no yog nyob ntawm hloov pauv ntawm pentose nyob rau hauv lub xub ntiag ntawm cov kua qaub kub rau furfural uas ces reacts nrog orcinol kom tawm xim ntsuab. Cov xim siv tau tuaj yeem ntsuas ntawm 665 nm.
orcinol reagent ua haujlwm li cas?
Thaum pentose tau rhuab nrog cov concentrated hydrochloric acid, nwsforms furfural. Orcinol reacts nrog cov furfural no muab cov xim ntsuab xim. Ferric chloride ua raws li cov catalyst. Tsuas yog purine nucleotides muab kev nyeem ntawv tseem ceeb.