Mesenchymal Stem Cell Isolation los ntawm Umbilical Cord
- Tshuaj qaum (UCs) hauv cov tshuaj hypochlorite (1:3).
- Yuav UCs nrog PBS.
- Tau UCs hauv 10% FBS / DMEM-qabzib tsawg txog li 12 teev.
- Tshuaj UCs peb zaug nrog PBS.
- Txhaj cov leeg thiab cov hlab ntsha nrog 3mL 0.1% collagenase hauv PBS.
- Cub rau 20 feeb ntawm 37 ° C.
Yuav ua li cas thiaj tau mesenchymal qia hlwb?
Mesenchymal qia hlwb yog cov neeg laus qia hlwb cais los ntawm ntau qhov chaw uas tuaj yeem sib txawv rau lwm hom hlwb. Hauv tib neeg, cov peev txheej no suav nrog; pob txha pob txha, rog (cov ntaub so ntswg adipose), umbilical qaum ntaub so ntswg (Wharton's Jelly) los yog kua amniotic (cov kua nyob ib puag ncig tus me nyuam hauv plab).
Yuav ua li cas cais mesenchymal qia hlwb?
MSCs feem ntau yog cais tawm raws li cov neeg nyob hauv cov yas-adherent cell siv cov txheej txheem yooj yim uas muaj cov ntaub so ntswg mincing, xaiv enzymatic digestion thiab cell outgrowth ntawm ib tug yas nto. Cov txheej txheem feem ntau tuaj yeem muab faib ua enzymatic thiab explant raws tu qauv.
Koj puas tuaj yeem tshem tawm koj tus kheej qia hlwb?
Autologous txhais tau tias koj cov qia hlwb yuav raug sau, khaws cia, thiab muab rov qab rau koj tom qab. Cov ntshav peripheral yog cov ntshav uas ncig hauv koj cov hlab ntsha. Tom qab koj cov qia hlwb raug sau, koj yuav tau txais tshuaj kho mob siab heev los tua cov qog nqaij hlav cancer hauv koj lub cev.
Yuav ua li cas rho cov qia hlwb?
Txoj kev tshaj plaws los sau cov qia hlwb yog tshem tawm cov ntshav tawm ntawm lub cev ib ntus, cais cov qia hlwb tawm, thiab tom qab ntawd rov qab cov ntshav mus rau lub cev. Txhawm rau txhawb cov qia hlwb hauv cov ntshav, cov tshuaj uas txhawb nqa lawv cov khoom yuav raug muab rau li 4 hnub ua ntej.
