2024 Tus sau: Elizabeth Oswald | [email protected]. Kawg hloov kho: 2024-01-13 00:07
Chromatography feem ntau yog siv los cais cov analytes los ntawm matrix thiab txiav txim siab txhua tus analytes cais. Feem ntau cov qauv yuav tsum tau kho - qauv kev npaj - ua ntej peb tuaj yeem pib sib cais chromatographic. Chromatography ua kom muaj txiaj ntsig zoo (nrhiav thiab txheeb xyuas) thiab kev tshuaj xyuas ntau.
Yuav ua li cas yog chromatography quantitative?
Kev ntsuas ntau uas siv chromatographic tsom xam yog raws li kev ntsuas ntawm qhov siab siab lossis qhov siab ntawm qhov siab ntawm lub ncov los ntawm cov qauv uas tsis paub qhov concentration. Txoj kev tshuaj ntsuam xyuas yuav tsum tau ua kom tiav ua ntej siv.
Kev tshuaj ntsuam zoo hauv chromatography yog dab tsi?
Cov ntaub ntawv zoo tau muab rho tawm los ntawm kev tshuaj ntsuam chromatographic yog txhawj xeeb rau kev txheeb xyuas cov khoom sib xyaw los ntawm kev sib piv ntawm lub sijhawm khaws cia ntawm lub ncov (tsawg lossis txheeb ze) nrog cov ntawm cov qauv (yog muaj), txhaj nyob rau hauv tib yam kev ntsuas (Fig. 5A).
Puas TLC chromatography quantitative?
Thin-layer chromatography (TLC) yog siv dav tshwj xeeb rau kev tshuaj xyuas tshuaj thiab zaub mov. Thaum muaj ntau phau ntawv hais txog kev txheeb xyuas qhov zoo ntawm cov tshuaj lom neeg los ntawm TLC, qhov tshwj xeeb tsom ntawm no yog ntawm kev tshuaj xyuas ntau.
Vim li cas roj chromatography quantitative?
Kev tshuaj xyuas ntau yuav tsum tau siv ob peb yam yooj yimcov cuab yeej txheeb cais. … Cov haujlwm tseem ceeb uas tus kws tshuaj ntsuam yuav tsum tau ua los ua kom muaj nuj nqis los ntawm cov roj chromatography yog qhov ntsuas ntawm tus qauv loj txhaj, kev txiav txim siab ntawm cov lus teb, thiab kev ntsuas ntawm thaj chaw ncov.
Pom zoo:
Puas yog qhov tsis zoo ntawm ntau qhov ntau lossis cov lus?
Lub npe tsis zoo tuaj yeem suav tau lossis suav tsis tau. Feem ntau, feem ntau siv, cov ntsiab lus, daim ntawv ntau ntau kuj yuav yog qhov tsis zoo. Txawm li cas los xij, hauv cov ntsiab lus tshwj xeeb,ntau daim ntawv tuaj yeem ua tsis tiavpiv txwv li.
Puas yog lo lus cov khoom siv feem ntau lossis ntau tshaj?
Cov khoom siv tuaj yeem suav tau lossis suav tsis tau. Feem ntau, feem ntau siv, cov ntsiab lus, ntau daim ntawv yuav kuj yog cov khoom siv. Txawm li cas los xij, hauv cov ntsiab lus tshwj xeeb, daim ntawv ntau ntau kuj tuaj yeem yog cov ntaub ntawv xws li.
Puas yog qhov zoo thiab zoo nkauj yog cov ntaub ntawv kawm zoo?
Qhov no yog ib qho kev kawm pheej yig heev thiab pheej yig dua me ntsis ntau dua li cov ntaub ntawv kawm hauv tsev. Lub Hom Phiaj Zoo thiab Zoo Nkauj yog txhawm rau ntxiv dag zog rau menyuam yaus thiab tsev neeg, yog vim li cas lawv khaws lawv cov txiaj ntsig qis.
Puas yog daim ntawv nug zoo lossis kev tshawb fawb ntau?
Kev Tshawb Fawb (cov lus nug) feem ntau tuaj yeem muaj ob qho lus nug ntau thiab zoo. Cov lus nug ntau yuav siv daim ntawv yog / tsis yog, lossis ntsuas qhov ntsuas (1 txog 5), thaum cov lus nug zoo yuav nthuav tawm lub thawv uas tib neeg tuaj yeem sau lawv cov lus.
Thaum twg yog chromatography cov txheej txheem sib cais zoo tshaj plaws?
Cov txheej txheem chromatography raws li kev faib tawm tau zoo heev ntawm kev sib cais, thiab txheeb xyuas cov molecules me me li amino acids, carbohydrates, thiab fatty acids. Txawm li cas los xij, affinity chromatographies (ie. ion-exchange chromatography) muaj txiaj ntsig zoo dua hauv kev sib cais ntawm macromolecules li nucleic acids, thiab proteins.
