2024 Tus sau: Elizabeth Oswald | [email protected]. Kawg hloov kho: 2024-01-13 00:07
Reverse transcriptase thawj zaug sau cov khoom sib txuas ntawm DNA los ua RNA: DNA hybrid. Tom ntej no, thim rov qab transcriptase los yog RNase H degrades RNA strand ntawm hybrid. Lub ib leeg-stranded DNA yog siv los ua tus qauv rau kev sib txuas ob-stranded DNA (cDNA).
Puas thim rov qab transcriptase ua haujlwm ntawm DNA?
Molecular biology
Cov txheej txheem PCR classical tuaj yeem siv rau DNA strands nkaus xwb, tab sis, nrog kev pab cuam rov qab transcriptase, RNA tuaj yeem hloov mus rau DNA, yog li ua PCR tsom xam ntawm RNA molecules ua tau. Rov qab transcriptase kuj tseem siv los tsim cDNA cov tsev qiv ntawv los ntawm mRNA.
Yuav rov qab cov ntaub ntawv rov qab siv mRNA li cas?
(b) Thib ob, enzyme thim rov qab transcriptase synthesizes strands ntawm DNA siv mRNA molecules ua qauv.
Tus qauv rau kev hloov pauv rov qab yog dab tsi?
Reverse transcriptase yog RNA-dependent DNA polymerase, catalyzing DNA synthesis siv RNA ua tus qauv. Cov khoom kawg yog hu ua complementary DNA (cDNA).
Puas thim rov qab transcriptase siv DNA primer?
Reverse transcriptase enzymes (RT) hloov ib leeg-stranded retroviral RNA genomes rau hauv ob-stranded DNA. RT enzyme tuaj yeem siv RNA thiab DNA primers, qhov qub tau siv tshwj xeeb thaum pib ntawm kev rho tawm- thiab ntxiv-strand synthesis.
Pom zoo:
Cov tshuaj twg yog nucleoside thim rov qab transcriptase inhibitors?
Available NRTIs zidovudine (Retrovir) Ylamivudine (Epivir) abacavir sulfate (Ziagen) didanosine (Videx) delayed-tso didanosine (Videx EC) stavudine (Zerit) emtricitabine (Emtriva) tenofovir disoproxil fumarate (Viread) Dab tsi yog nucleoside thim rov qab transcriptase enzyme inhibitors?
Vim li cas nws tseem ceeb rau tus qauv kev tshawb fawb kom thim rov qab?
Raws li txhua yam tshuaj tiv thaiv, kev hloov pauv rov qab pab piav qhia qee yam uas peb pom tshwm sim hauv ntiaj teb ib puag ncig peb. Txhua lub sij hawm cov me nyuam yaj, khov los yog (hauv lub neej tom ntej) boil ib yam dab tsi, lawv yuav muaj peev xwm paub txog qee degree txheej txheem dab tsi tso cai rau nws tshwm sim.
Puas thim rov qab transcriptase inhibitors kho mob HIV?
HIV kho tsis tau, tab sis feem ntau tswj tau. Kev kho nrog nucleoside/nucleotide thim rov qab transcriptase inhibitors (NRTIs) yog ib txoj hauv kev los pab tiv thaiv tus kab mob los ntawm kev rov ua dua thiab tswj kev kis kab mob HIV. Puas thim rov qab transcriptase inhibitors kho HIV?
Puas thim rov qab transcriptase puas muaj ntawv pov thawj?
Reverse transcriptase (RT) enzymes luam RNA rau hauv DNA. Tsis zoo li ntau DNA polymerases, RT enzymes tsis muaj cov ntawv pov thawj uas kuaj xyuasrau qhov tsis raug hauv DNA tshiab. Puas thim rov qab transcriptase muaj kev ua haujlwm kho?
Puas yog thim rov qab transcriptase ib qho kev txwv enzyme?
Reverse transcriptase yog ib qho enzyme uas tsim los ntawm qee cov kab mob (retroviruses, uas khaws lawv cov ntaub ntawv caj ces li RNA tsis yog DNA), uas, raws li lub npe txhais, thim rov qab cov txheej txheem ntawm kev sau npe. Cov piv txwv ntawm kev txwv enzymes yog dab tsi?