Reverse transcriptase thawj zaug sau cov khoom sib txuas ntawm DNA los ua RNA: DNA hybrid. Tom ntej no, thim rov qab transcriptase los yog RNase H degrades RNA strand ntawm hybrid. Lub ib leeg-stranded DNA yog siv los ua tus qauv rau kev sib txuas ob-stranded DNA (cDNA).
Puas thim rov qab transcriptase ua haujlwm ntawm DNA?
Molecular biology
Cov txheej txheem PCR classical tuaj yeem siv rau DNA strands nkaus xwb, tab sis, nrog kev pab cuam rov qab transcriptase, RNA tuaj yeem hloov mus rau DNA, yog li ua PCR tsom xam ntawm RNA molecules ua tau. Rov qab transcriptase kuj tseem siv los tsim cDNA cov tsev qiv ntawv los ntawm mRNA.
Yuav rov qab cov ntaub ntawv rov qab siv mRNA li cas?
(b) Thib ob, enzyme thim rov qab transcriptase synthesizes strands ntawm DNA siv mRNA molecules ua qauv.
Tus qauv rau kev hloov pauv rov qab yog dab tsi?
Reverse transcriptase yog RNA-dependent DNA polymerase, catalyzing DNA synthesis siv RNA ua tus qauv. Cov khoom kawg yog hu ua complementary DNA (cDNA).
Puas thim rov qab transcriptase siv DNA primer?
Reverse transcriptase enzymes (RT) hloov ib leeg-stranded retroviral RNA genomes rau hauv ob-stranded DNA. RT enzyme tuaj yeem siv RNA thiab DNA primers, qhov qub tau siv tshwj xeeb thaum pib ntawm kev rho tawm- thiab ntxiv-strand synthesis.
