Peptide daim ntawv cog lus; uncharged tRNA txav mus rau qhov E site & tom qab tawm ntawm ribosome; lub mRNA tau hloov pauv 3 lub hauv paus mus rau sab laug, ua rau tRNA bearing dipeptide hloov mus rau P site P site P-site (rau peptidyl) yog qhov thib ob khi qhov chaw rau tRNA hauv ribosome. Lwm ob qhov chaw yog A-site (aminoacyl), uas yog thawj qhov chaw khi hauv ribosome, thiab E-site (tawm), qhov thib peb. Thaum lub sij hawm txhais lus protein, P-site tuav lub tRNA uas txuas nrog cov saw hlau loj hlob. https://en.wikipedia.org › wiki › P-site
P-site - Wikipedia
. 3. Thawj kauj ruam ntawm elongation tiav, yooj yim los ntawm EF-G.
Qhov chaw twg ntawm ribosome tuav ib qho tRNA uas tsis tau them?
Cov saw hlau loj hlob polypeptide raug xa mus rau qhov kawg ntawm cov amino acid uas tuaj, thiab A-site tRNA tuav ib ntus tuav cov saw hlau loj hlob, thaum P-site tRNA Tam sim no khoob lossis tsis tau them.
Dab tsi ua rau ejection ntawm uncharged tRNA los ntawm E site ntawm ribosome thaum lub sij hawm txhais lus?
Aminoacyl-tRNA Binding (Cov Nqi Zog=1 GTP) Aminoacyl-tRNA tsis tuaj yeem khi kom txog thaum muaj kev sib khi ua ke nrog cov khoom elongation EF-Tu thiab GTP (guanosine triphosphate). Kev khi ntawm qhov nyuaj no ua rau ejection ntawm deacylated tRNA los ntawm E site.
Yuav ua li cas tRNAs txuas rau qhov tseeb amino acid?
Yuav ua li cas cov aminoacid ua txuas rau tRNA? Lub zog ntawm ATP hydrolysis yog siv txhawm rau txuas txhua cov amino acid rau nws cov tRNA molecule hauv kev sib txuas ntawm lub zog siab.
4 kauj ruam ntawm kev txhais lus yog dab tsi?
Kev txhais lus tshwm sim nyob rau plaub theem: ua kom (npaj txhij), pib (pib), elongation (ua kom ntev) thiab txiav (nres). Cov ntsiab lus no piav qhia txog kev loj hlob ntawm cov amino acid saw (polypeptide). Amino acids raug coj mus rau ribosomes thiab sib sau ua ke rau hauv cov protein.
