Electrophoresis ua rau koj paub qhov txawv DNA ntawm qhov ntev sib txawv. DNA raug nqi tsis zoo, yog li ntawd, thaum muaj hluav taws xob tam sim no siv rau gel, DNA yuav tsiv mus rau tus nqi electrode zoo. … Lawv yuav tshwm sim raws li ib pawg ntawm cov gel.
Puas DNA txav mus rau cathode lossis anode?
Cov pob txha phosphate ntawm DNA (thiab RNA) molecule yog qhov tsis zoo, yog li thaum muab tso rau hauv qhov hluav taws xob, DNA tawg yuav tsiv mus rau zoo them anode.
DNA fragments migrate nyob qhov twg thaum electrophoresis?
DNA tawg yog qhov tsis zoo, yog li lawv txav mus rau qhov zoo electrode. Vim hais tias tag nrho cov DNA seem muaj tib qhov nqi ntawm ib pawg, cov khoom me me txav mus los ntawm cov gel sai dua cov loj.
Vim li cas DNA txav mus rau cathode thaum electrophoresis?
Cov khoom siv hluav taws xob tuaj yeem sib cais vim tias lawv tsiv mus rau qhov sib txawv ntawm cov gel. Cov khoom tsis zoo ib txwm txav mus rau ntawm tus ncej zoo whereas cov khoom muaj txiaj ntsig zoo ib txwm txav mus rau ntawm tus ncej tsis zoo (tawm tsam nyiam).
DNA migrate thiab daws li cas hauv gel electrophoresis?
(a) Cov DNA tawg tau daws raws li lawv qhov loj me los ntawm cov nyhuv sieving muab los ntawm agarose gel. Yog li ntawd, qhov me me ntawm qhov me me, qhov deb nws txav mus. (b) Covmuab agarose gel electrophoresis qhia tias kev tsiv teb tsaws ntawm DNA tawg tsis zoo nyob rau hauv txoj kab 1 thiab zom cov DNA seem hauv txoj kab 2 txog 4.
