txheej txheem:
- Tshem tawm cov crystalline phenol los ntawm -20 ° C lub freezer thiab thaw nws ntawm 60-65 ° C.
- Ntxiv cov ntim xav tau ntawm phenol rau hauv lub raj mis uas tsim nyog. …
- Ntxiv qhov ntim sib npaug ntawm 10X TE rau phenol.
- Yuav ua kom muaj zog thiab cia cov khaubncaws sab nraud povtseg.
- Tshem tawm cov dej ntws tawm (sab saum toj) txheej.
Koj ua licas phenols li cas?
Noj 100g phenol lub raj mis rau fume hood, qhib nws, thiab ncuav rau ~ 100 ml 50 mM TrisCl pH 8. Kaw lub hau kom nruj thiab co maj mam. Cia sawv ntsug rau ib teev lossis ob zaug kom txog thaum cov phenol liquifies thiab cov theem sib cais. Tshem cov supernatant nrog lub pipette (pov tseg rau hauv 'chlorinated solvent khib nyiab' thawv).
Koj ua li cas thiaj ua tau phenol saturated?
Acid phenol- Rau cov khoom siv phenol ntxiv RNase-dawb dej kom txog thaum muaj txheej txheej dej saum cov phenol: Kub lub raj mis tshiab (500g) txog 65 oC, tawg lub hau. Ntxiv 100 ml ntawm RNase-dawb dej. Sib tov thiab cia txias. Ntxiv li 100 mls dej ntxiv kom txog rau thaum cov dej me ntsis nyob rau saum cov phenol thiaj li yog dej saturated.
YDab tsi yog molarity ntawm phenol?
Tus Molarity yog ~10.6. Qhov ceev ntawm phenol yog 1.05g / ml, thiab qhov hnyav molecular yog 94.11.
Koj khaws cov phenol li cas?
Kawm hauv ib lub thawv nruj kaw. Khaws rau hauv qhov chaw txias, qhuav, qhov cua kom deb ntawm qhov chaw kub lossis hluav taws kub. Tiv thaiv kev puas tsuaj rau lub cev. Khwcais los ntawm cov khoom siv hluav taws xob lossis cov khoom sib txuas, thiab tawm ntawm tshav ntuj ncaj qha.
