2024 Tus sau: Elizabeth Oswald | [email protected]. Kawg hloov kho: 2024-01-13 00:07
txheej txheem:
- Tshem tawm cov crystalline phenol los ntawm -20 ° C lub freezer thiab thaw nws ntawm 60-65 ° C.
- Ntxiv cov ntim xav tau ntawm phenol rau hauv lub raj mis uas tsim nyog. …
- Ntxiv qhov ntim sib npaug ntawm 10X TE rau phenol.
- Yuav ua kom muaj zog thiab cia cov khaubncaws sab nraud povtseg.
- Tshem tawm cov dej ntws tawm (sab saum toj) txheej.
Koj ua licas phenols li cas?
Noj 100g phenol lub raj mis rau fume hood, qhib nws, thiab ncuav rau ~ 100 ml 50 mM TrisCl pH 8. Kaw lub hau kom nruj thiab co maj mam. Cia sawv ntsug rau ib teev lossis ob zaug kom txog thaum cov phenol liquifies thiab cov theem sib cais. Tshem cov supernatant nrog lub pipette (pov tseg rau hauv 'chlorinated solvent khib nyiab' thawv).
Koj ua li cas thiaj ua tau phenol saturated?
Acid phenol- Rau cov khoom siv phenol ntxiv RNase-dawb dej kom txog thaum muaj txheej txheej dej saum cov phenol: Kub lub raj mis tshiab (500g) txog 65 oC, tawg lub hau. Ntxiv 100 ml ntawm RNase-dawb dej. Sib tov thiab cia txias. Ntxiv li 100 mls dej ntxiv kom txog rau thaum cov dej me ntsis nyob rau saum cov phenol thiaj li yog dej saturated.
YDab tsi yog molarity ntawm phenol?
Tus Molarity yog ~10.6. Qhov ceev ntawm phenol yog 1.05g / ml, thiab qhov hnyav molecular yog 94.11.
Koj khaws cov phenol li cas?
Kawm hauv ib lub thawv nruj kaw. Khaws rau hauv qhov chaw txias, qhuav, qhov cua kom deb ntawm qhov chaw kub lossis hluav taws kub. Tiv thaiv kev puas tsuaj rau lub cev. Khwcais los ntawm cov khoom siv hluav taws xob lossis cov khoom sib txuas, thiab tawm ntawm tshav ntuj ncaj qha.
Pom zoo:
Los ntawm cov khoom sib npaug ntawm kev sib npaug?
Cov cuab yeej sib luag ntawm kev sib luag hauv paus hais uas ob sab ntawm kev sib npaug yog tib yam. Qhov no ua rau kev txiav txim siab vim tias thaum ib yam dab tsi sib luag, nws yog tib yam ntawm ob sab. Cov cuab yeej sib luag ntawm kev sib npaug tso cai rau sab laug ntawm qhov sib npaug los ua sab xis thiab rov ua dua.
Thaum tus lej sib npaug y yog tus lej sib npaug?
Thaum muab tus lej Y yog tus lej sib npaug X=xn – 1xn-2…. x0 siv me ntsis-pair recoding hauv Booth's algorithm, ib nrab cov khoom tsim tawm raws li cov lus hauv qab no. Nqe lus piav qhia: ALU tsis tuaj yeem faib cov lej ncaj qha, nws tsuas tuaj yeem ntxiv, rho tawm lossis ua haujlwm.
Yuav tsum sib npaug sib npaug siab lossis qis?
A siab dua qhov sib npaug feem ntau qhia tias muaj kev pheej hmoo tsawg dua thiab muaj zog nyiaj txiag ntau dua li qhov qis dua. Yog tias lub tuam txhab qhov sib npaug sib npaug siab, nws yuav nyiaj ntau dua ntawm nws cov cuab tam nrog kev ncaj ncees thiab ib feem qis nrog cov nuj nqis.
Puas yog cov nqi sib npaug sib npaug?
Kev sib npaug ntawm thaj av, zoo li kev them nyiaj tshaj ib feem ntawm thaj av, tuaj yeem hla dhau raws li txoj cai yog tias muaj kev muag khoom raws li txoj cai. Puas yog tus nqi sib npaug yog tus nqi sau npe? Tus nqi sib npaug tsis tuaj yeem sau npe rau ntawm nws tus kheej txoj cai thiab yuav tsum tau tiv thaiv los ntawm kev nkag mus rau hauv daim ntawv ceeb toom.
Puas trs cables sib npaug lossis tsis sib npaug?
TRS cables tuaj yeem siv rau mono, ntsuas cov cim ntsuas nrog rau cov cim tso suab. Ib qho piv txwv ntawm mono, cov teeb liab sib npaug yuav yog kab tawm tswv yim lossis cov zis tawm los ntawm cov khoom sib tov lossis lub suab interface. Lub mloog pob ntseg tau txais cov cim tso suab ntawm TRS cables.
