UV ntes yog ib qho khoom siv ntau heev rau HPLC tsom xam. Thaum lub sij hawm soj ntsuam, cov qauv mus los ntawm cov xim ntshiab-tsawg iav cell, hu ua flow cell. Thaum UV teeb yog irradiated ntawm lub xovtooj ntawm tes, cov qauv absorbs ib feem ntawm UV teeb.
Muaj pes tsawg hom HPLC ntes muaj?
Lawv yog ntawm peb hom, piv txwv li cov ntsuas qhov ntsuas wavelength ruaj khov, ntsuas qhov sib txawv wavelength thiab cov diode array detectors.
Vim li cas UV ntes siv hauv HPLC?
HPLC UV ntes tau siv nrog kev ua haujlwm siab ua kua chromatography txhawm rau txheeb xyuas thiab txheeb xyuas cov ntsuas hauv cov qauv. Lub UV pom HPLC ntes siv teeb los tshuaj xyuas cov qauv. Los ntawm kev ntsuas tus qauv qhov nqus ntawm lub teeb ntawm qhov sib txawv wavelengths, cov tshuaj ntsuam xyuas tuaj yeem txheeb xyuas.
Dab tsi yog qhov ntsuas thoob ntiaj teb hauv HPLC?
A universal detector txhais tau tias yog ib qho uas 'tuaj yeem teb rau txhua qhov khoom hauv kem cov dej ntws tawm tsuas yog theem txawb' 2. Nyob rau hauv sib piv, xaiv detectors yog txhais tau tias yog 'detectors uas teb rau ib tug muaj feem xyuam rau cov piv txwv ntawm cov piv txwv Cheebtsam nyob rau hauv kem effluent'.
Dab tsi yog cov cuab yeej siv hauv chromatography?
UV/Vis spectrometers thiab UV detectors yog cov khoom siv chromatography tshaj plaws. Lwm txoj hauv kev tshawb nrhiav kuj tseem ua tau xws li conductivity, pH, refractive index, lub teeb tawg, fluorescence thiab radioactivity detection.
