Cov tshuaj tiv thaiv acid-hydrolysis nrog 6 M HCl ua rau cov dej sib ntxiv rau txhua covalent peptide daim ntawv cog lus, yielding tus neeg xav tau cov amino acids (Daim duab 1). Txawm li cas los xij, tsis yog tag nrho cov amino acids tau rov qab los ntawm hydrolysis los ntawm HCl.
Yuav ua li cas hydrolyze peptide bonds?
Kev poob qis. Ib daim ntawv cog lus peptide tuaj yeem tawg los ntawm hydrolysis (tus ntxiv dej). Nyob rau hauv lub xub ntiag ntawm dej lawv yuav zom thiab tso 8-16 kilojoule / mol (2-4 kcal / mol) ntawm Gibbs zog. Cov txheej txheem no qeeb heev, nrog rau ib nrab lub neej ntawm 25 ° C ntawm nruab nrab ntawm 350 thiab 600 xyoo ib daim ntawv cog lus.
HCl puas peptide bonds?
Peptide bonds yog cleaved los ntawm kev ua ntawm HCl/trifluoroacetic acid (TFA) vapor sib tov. Kev sib kis rau cov dej sib tov yog txo kom tsawg (1-3 pmol). Rov qab ntawm hydrophobic amino acid yog txhim kho. Lub sijhawm luv luv tau ua tiav thiab tshem tawm cov kua qaub sai sai.
Puas kua qaub tawg peptide bonds?
Peb tuaj yeem rhuav tshem peptide bonds (amide txuas) koom nrog cov amino acid units ua kelos ntawm kev siv dej acidified hauv cov tshuaj tiv thaiv acid hydrolysis. … Qhov no cuam tshuam cov qauv tseem ceeb ntawm cov protein thiab cov protein ua rau cov khoom me me, thaum kawg ua rau ntau cov amino acids.
Puas yog acid hydrolysis ntawm peptide bonds tshwm sim hauv plab?
Lub plab ua kom ua haujlwmthiab pib cov proteinthiab lipid hydrolysis. Peptides, amino acids, thiab fatty acids tso tawm nyob rau hauv cov txheej txheem no synchronize cov kua txiv pancreatic thiab cov kua tsib rau hauv cov hnyuv. Kwv yees li 2 L ntawm cov kua txiv hmab txiv ntoo yog tsim txhua hnub, muaj ob peb yam tseem ceeb.
