Thaum twg koj dilute cov tshuaj tiv thaiv kab mob, sib tov maj mam kom ntseeg tau tias muaj kev sib xyaw ua ke. Peb pom zoo tawm tsamsiv lub tshuab vortex, vim tias vortexing tuaj yeem ua rau tsis ua haujlwm ntawm cov tshuaj tiv thaiv.
Koj puas tuaj yeem tig cov tshuaj tiv thaiv?
Rau ntau ntawm peb cov tshuaj tiv thaiv kab mob, khov ntawm -20 ° C lossis -80 ° C hauv cov khoom me me yog qhov kev cia zoo. … Thaum tau txais cov tshuaj tiv thaiv, centrifuge ntawm 10, 000 x g rau 20 vib nas this kom rub cov tshuaj uas tau daig hauv cov xov ntawm lub vial, thiab hloov aliquots mus rau hauv cov raj uas muaj protein ntau-binding microcentrifuge.
Kuv Yuav Tsum Muaj Vortex enzyme?
Tsis txhob vortex enzyme cov tshuaj tiv thaiv . Koj yuav tsum tau lub cev sib xyaw cov enzyme rau hauv cov tshuaj tiv thaiv vim tias cov enzyme muaj nyob rau hauv cov tshuaj glycerol uas yuav tso rau hauv lub cev. hauv qab ntawm koj cov tshuaj tiv thaiv. Ua li no los ntawm kev maj mam muab tso rau hauv thiab rub lub raj nrog koj tus ntiv tes.
Koj puas tuaj yeem vortex proteins?
Tsis txhob VORTEX koj cov protein. Tsis tas li ntawd, tsis txhob sonicate los yog pipet vigorously (mus rau ib tug taw tes uas muaj npuas nyob rau hauv koj tus qauv). Proteins hauv cov tshuaj tsis nyiam huab cua los yog shear stress.
Yog vortex enzymes nyab xeeb?
Raws li txoj cai ntawm tus ntiv tes xoo, tsis txhob vortex cov tshuaj uas muaj cov protein uas muaj cov haujlwm tseem ceeb(xws li enzyme, tshuaj tiv thaiv kab mob).
31 cov lus nug ntsig txog pom
Kuv Puas Muaj Vortex DNTP?
Tsis txhob vortex PCR mix. Ntxiv DNA polymerase (Taq) rau cov tshuaj tiv thaiv tube kawg. … Zamoverloading PCR cov khoom rau hauv gel; qhov no yuav ua rau muaj kev kis kab mob sib kis lossis txhais lus tsis raug ntawm cov txiaj ntsig.
Puas yog vortex primers?
Tsis txhob vortex ntau. Tab sis koj tuaj yeem vortex maj mam rau 5-10 rau kev sib xyaw kom zoo. Nws yuav tsis tawg primers.
Koj puas tuaj yeem tiv thaiv vortex?
Thaum twg koj dilute cov tshuaj tiv thaiv kab mob, sib tov maj mam kom ntseeg tau tias muaj kev sib xyaw ua ke. Peb pom zoo kom tsis txhob siv lub vortex tov khoom, raws li vortexing may pab txhawb rau inactivation ntawm cov tshuaj tiv thaiv.
Qhov txawv ntawm Vortex thiab centrifuge yog dab tsi?
A vortex yog ib qho kev cuam tshuam hauv zos uas tshwm sim los ntawm turbulent flow. … Lub nrawm ntawm lub vortex ntau dua nyob ze ntawm lub axis ntawm kev sib hloov thiab txo mus rau ntawm ntug ntawm lub vortex. Tsis zoo li lub vortex, lub centrifuge tsim centrifugal quab yuam perpendicular mus rau lub axis tsau ntawm spin.
Vim li cas Vortex phem rau kev sib xyaw?
Vortexing tag nrho cov ntsiab lus ntawm lub nkoj yog hais txog txoj kev phem tshaj plaws ntawm kev sib xyaw kom zoo ib yam. Lub vortex nyob rau hauv lub thawv cylindrical zoo yuav ua rau lub cev sib hloov ntawm cov kua, uas yuav luag tsis muaj radial lossis ntsug ntws, ob qho tib si tsim nyog los ua kom tiav kev sib xyaw.
Koj puas tuaj yeem vortex SYBR?
I wouldn't recommend vortexing SYBR Ntsuab, ua ntej los yog tom qab ua tus tswv mix. Kuv luv luv vortex thiab tig down lub primer ntawm kev txaus siab, sib tov tag nrho cov tswv-sib xyaw (nrog sybr) los ntawm inversion, thiab ua ib tug ceev tig down (ib tug mem tes los yog li ntawd). Vortexing tom qab ntxiv Sybr ua rau muaj kev pheej hmoo ntawm kev puas tsuaj rau Taq pol.
Koj Puas Tau Vortexkab mob?
Cov kab mob hauv cov tee dej spontaneously tsimbi-directional vortex, nrog cov kab mob nyob ze ntawm qhov chaw ntawm qhov poob ua luam dej nyob rau sab nraud ntawm cov kab mob ua luam dej ze ntawm ntug. … cov kab mob subtilis nyob hauv ib tee dej, ib qho txawv heev tshwm sim.
Puas yog vortex hlwb?
Vortexing ntawm cov hlwb yuav ua rau lawv puas tsuaj, tab sis tus nqi ntawm kev puas tsuaj thiab tus nqi ntawm cov hlwb puas los ntawm vortexing yog heev nyob ntawm seb hom cell. … Txawm li cas los xij peb tsis muaj vortex hlwb raws li peb lub xovtooj tseem ceeb heev rau kev ntxhov siab.
Yuav ua li cas yog tias koj khov tshuaj tiv thaiv kab mob?
Rov qab khov / thawvoj voog ua rau cov tshuaj tiv thaiv kab mob[4]. Txawm li cas los xij nws yog ib qho tseem ceeb uas cov tshuaj tiv thaiv polyclonal khaws cia yuav tsum nyob rau hauv siab concentration. Monoclonal antibodies tuaj yeem khaws cia ntawm -20 ° C hauv 50% glycerol.
Koj khaws cov tshuaj tiv thaiv li cas?
Cov tshuaj tiv thaiv yog cov protein thiab yuav tsum yog khaws cia txias (txias, hauv dej khov, lossis khov) thaum tsis siv. c. Qhov ntau dilute antibody yog, qhov tsis ruaj khov. Yog li ntawd, nws yog ib lub tswv yim zoo los khaws cov tshuaj tiv thaiv kab mob hauv cov ntaub ntawv uas tsis muaj dilution.
Kuv puas tuaj yeem khov cov tshuaj tiv thaiv kab mob?
Rov qab khov / thaw cycles tuaj yeem ua rau cov tshuaj tiv thaiv kab mob, ua rau nws tsim cov sib sau ua ke uas txo cov tshuaj tiv thaiv kab mob. Khaws ntawm -20 ° C yuav tsum tsim nyog rau feem ntau cov tshuaj tiv thaiv; Tsis muaj qhov pom zoo kom khaws cia ntawm -80 ° C.
Vortexing yog dab tsi?
Vortexing yog thaum lub twj tso kua mis pib nqus cua los ntawm cov kua dej,tsim ib lub vortex pom nyob rau saum cov kua. Qhov no tsim kom muaj huab cua thiab cov kua dej nkag mus rau hauv lub twj tso kua mis uas ua rau muaj kev kub ntxhov, ua rau txo qis ntawm qhov hluav taws xob ntawm lub twj tso kua mis.
Centrifuge puas siv tau rau kev sib tov?
centrifugation ntawm Percoll® cov txiaj ntsig hauv kev ntom ntom ntawm kev ua kom nruj vim qhov ntau thiab tsawg hauv nruab nrab. Percoll® tuaj yeem siv tau rau tsim ntawm gradientsyog los ntawm kev siv cov khoom siv gradient los yog los ntawm kev kub ceev centrifugation.
Pob cov tshuaj tiv thaiv monoclonal yuav tsum tau muab tso rau hauv tub yees?
Cov tshuaj tiv thaiv kab mob feem ntau muaj zog heev thiab yuav tsum khaws cov haujlwm ua haujlwm yog khaws cia hauv tub yees ntawm 2-8oC txog li 12 lub hlis. Rau lub sij hawm ntev cia, nws raug nquahu kom khaws cov khoom khov rau hauv me me aliquots. Tsis txhob rov ua kom khov thiab thawing voj voog, uas yuav cuam tshuam rau cov khoom siv.
Cov tshuaj tiv thaiv kab mob tuaj yeem nyob hauv chav sov ntev npaum li cas?
Raws li cov tshuaj tiv thaiv tshiab tau tsim, Cell Signaling Technology (CST) cov kws tshawb fawb sim cov tshuaj tiv thaiv kev ruaj ntseg thiab kev ua haujlwm tom qab lub sijhawm cia ntawm ntau tshaj 7 hnub ntawm -20 ° C, ntawm chav tsev kub thiab ntawm 37 ° C. Tag nrho cov tshuaj tiv thaiv kab mob tau kuaj pom tias muaj zog thiab ruaj khov thaum khaws cia hauv chav sov li ntawm dej khov.
Cov tshuaj tiv thaiv puas tuaj yeem khaws cia ntawm?
Feem ntau cov tshuaj tiv thaiv tuaj yeem khaws cia ntawm -20 °Cyam tsis muaj kev poob rau hauv lawv lub peev xwm khi. Nws yog qhov zoo tshaj kom tsis txhob khaws cov tshuaj tiv thaiv kab mob hauv lub freezer uas tsis muaj Frost.
Koj puas tuaj yeem vortex oligonucleotides?
Muajoligos kuj yooj yim rau resuspend; Txawm li cas los xij, cov hloov kho nrog fluorophores lossis hydrophobic molecules yuav xav tau sijhawm ntau dua los ua kom tiav solubilized. … Yog tias qhov kev rov ua dua tshiab nyuaj, sim ua kom sov lub oligo ntawm 55 ° C rau 1-5 feeb, ces vortex kom huv si.
Koj puas tuaj yeem vortex plasmid DNA?
Vortexing thiab pipetting koj plasmid - coj nws yooj yim !Nicked los yog linear DNA yuav tshwm sim vim mechanical shearing ntawm DNA yog tias preps yog vortexed los yog shaken heev. Thaum lub sij hawm kev sib cais ntawm plasmid. Yog li coj nws yooj yim heev; sib tov maj mam, tsis txhob vortex thiab pipette softly thiab sparingly.
TE buffer yog dab tsi?
TE Buffer, 1X, Qib Molecular (pH 8.0), yog qhov tsis muaj 10mM Tris-HCl muaj 1mM EDTA•Na2. Khoom: pH ntawm 25 ° C: 7.9-8.1.
