H&E yog kev sib xyaw ntawm ob qhov kev tshawb fawb keeb kwm: hematoxylin thiab eosin. Lub hematoxylin stains cell nuclei ib purplish xiav, thiab eosin stains lub extracellular matrix thiab cytoplasm liab, nrog rau lwm cov qauv noj ntawm ntau ntxoov, hues, thiab ua ke ntawm cov xim.
eosin staining siv rau dab tsi?
Eosin tuaj yeem siv rau stain cytoplasm, qe ntshav liab, collagen, thiab nqaij fibers rau kev kuaj mob. Nws feem ntau yog siv los ua cov tshuaj tiv thaiv rau hematoxylin hauv H&E staining.
H thiab E sawv cev rau dab tsi?
H&E sawv rau hematoxylin thiab eosin. Cov no yog ob lub stains uas feem ntau siv rau hauv cov qauv ntaub so ntswg kom lawv tuaj yeem pom hauv qab lub microscope.
Cov qauv ntawm tes ua li cas eosin thiab hematoxylin stain?
Hematoxylin precisely stains nuclear Cheebtsam, suav nrog heterochromatin thiab nucleoli, thaum eosin stains cytoplasmic Cheebtsam suav nrog collagen thiab elastic fibers, nqaij fibers thiab cov qe ntshav liab.
Dab tsi yog stain hauv paus ntsiab lus ntawm hematoxylin thiab eosin txheej txheem kev kho mob daim ntawv thov ntawm stain?
Hematoxylin thiab eosin yog cov txheej txheem stains siv rau kev ua qauv qhia ntawm nucleus thiab cytoplasmic inclusions. Alum ua raws li mordant thiab hematoxylin uas muaj alum stains nucleus lub teeb xiav uas tig liab nyob rau hauv muaj kua qaub. Kev sib txawv ntawm tes yog ua tiav los ntawm kev kho cov ntaub so ntswg nrog cov kua qaubsolution.
