2024 Tus sau: Elizabeth Oswald | [email protected]. Kawg hloov kho: 2024-01-13 00:07
Homology qauv kuj tseem siv tau txhawm rau txheeb xyuas qhov sib txawv me ntsis ntawm cov proteins uas tsis tau daws txhua qhov teeb meem . Piv txwv li, txoj kev tau siv los txheeb xyuas qhov chaw cation binding ntawm Na+/K+ ATPase thiab los tawm tswv yim txog qhov sib txawv ATPases 'binding affinity.
homology qauv siv rau dab tsi?
Homology qauv yog ib qho ntawm cov qauv kev kwv yees kwv yees uas yog siv los txiav txim siab protein 3D qauv los ntawm nws cov amino acid sequence. Nws raug suav hais tias yog qhov tseeb tshaj plaws ntawm cov qauv kev kwv yees kwv yees. Nws muaj ntau cov kauj ruam uas ncaj nraim thiab yooj yim rau kev thov.
homology qauv yog dab tsi thiab vim li cas nws thiaj xav tau?
Homology qauv tau txais peb qhov qauv qauv ntawm lub hom phiaj protein raws li qhov zoo sib xws ntawm cov qauv thiab lub hom phiaj ua ntu zusthiab cov txheej txheem no ua pov thawj kom ua tau zoo thaum nws los txog rau kev kawm membrane proteins uas nyuaj rau crystallize zoo li GPCR raws li nws muab ntau dua kev nkag siab ntawm …
Yuav ua li cas homology?
muaj ob peb kauj ruam koom nrog hauv homology qauv
- qauv xaiv siv koj lub hom phiaj ua ntu zus. Rau lub hom phiaj no koj tuaj yeem ua BLASTp tshawb nrhiav tawm tsam txhua yam muaj protein ntau (PDBs). …
- Txhim kho cov qauv thiab lub hom phiaj sib lawv liag. …
- Qhov zoo ntawm koj tus qauv. …
- Kev kho kom zoontawm koj tus qauv raws li.
Yuav ua li cas thiaj ua tau homology zoo?
Yog tias peb txhais "tus qauv ua tau zoo ntawm homology" raws li ib tus muaj <=2 Å rmsd los ntawm cov qauv empirical, ces tus qauv yuav tsum muaj >=60% ib ntus tus kheej nrog lub hom phiaj kom ua tiav tus nqi >70%. Txawm tias nyob rau ntawm cov cim qhia tau zoo (60%-95%), ntau npaum li ib ntawm kaum homology qauv muaj rmsd >5 Å vs.
Pom zoo:
Serial homology tshwm sim nyob qhov twg?
Vertebrates tau hloov kho vertebrae thiab tav nyob rau hauv ntau txoj kev; Cov vertebra tseem ceeb tau rov hais dua thiab hloov mus rau ntau hom pob txha: sacral, lumbar, thoracic, thiab ncauj tsev menyuam. Txawm li cas los xij, tej zaum ib qho piv txwv zoo tshaj plaws ntawm serial homology tuaj yeem pom nyob rau hauv lub cev tsim ntawm hom cray-fish.
Thaum twg siv los yog tsis siv?
Ib leeg: ib leeg txhais tau tias "ib ntawm ob"; tsis txhais hais tias "tsis muaj ntawm ob." Siv cov lus qhia ib leeg. Ib yam ua ke nrog lossis; tsis yog ua ke nrog los yog. Koj hais kuv los tsis yog kuv thiab? Tsis yog grammatically tseeb.
Thaum twg siv i.e. thiab thaum twg siv xws li?
I.e. yog ib qho luv luv rau kab lus id est, uas txhais tau tias "uas yog." I.e. yog siv los rov hais dua ib yam dab tsi uas tau hais yav dhau los txhawm rau kom meej nws lub ntsiab lus. E.g. luv luv rau exempli gratia, uas txhais tau tias "
Thaum twg siv polyacrylamide thiab thaum twg agarose?
Agarose gels siv nrog DNA, vim qhov loj ntawm biomolecules (DNA feem ntau yog ntau txhiab kDa). Rau cov protein gels, polyacrylamide muab daws teeb meem zoo, raws li qhov me me me (50 kDa yog qhov raug) zoo dua rau qhov sib txawv ntawm cov gel.
Thaum twg siv cov khoom siv?
Throughput yog hais txog ntau npaum li cas cov ntaub ntawv tuaj yeem xa los ntawm ib qhov chaw mus rau lwm qhov hauv lub sijhawm. Nws yog siv los ntsuas qhov kev ua tau zoo ntawm hard drives thiab RAM , nrog rau kev sib txuas hauv Is Taws Nem thiab network.
