Agarose gels siv nrog DNA, vim qhov loj ntawm biomolecules (DNA feem ntau yog ntau txhiab kDa). Rau cov protein gels, polyacrylamide muab daws teeb meem zoo, raws li qhov me me me (50 kDa yog qhov raug) zoo dua rau qhov sib txawv ntawm cov gel.
Qhov txawv ntawm agarose gel electrophoresis thiab polyacrylamide gel electrophoresis yog dab tsi?
Qhov sib txawv tseem ceeb ntawm agarose thiab polyacrylamide yog agarose yog siv rau hauv agarose gel electrophoresis(AGE) feem ntau rau kev sib cais ntawm DNA, hos polyacrylamide yog siv hauv polyacrylamide gel. electrophoresis (PAGE) feem ntau yog rau kev sib cais ntawm cov proteins.
Vim li cas polyacrylamide gels zoo dua agarose gel?
Polyacrylamide gels muaj peb yam tseem ceeb hauv qab no ntawm agarose gels: (1) Lawv qhov kev daws teeb meem zoo heev uas lawv tuaj yeem cais cov molecules ntawm DNA uas nws qhov ntev sib txawv li ntawm 0.1% (piv txwv li, 1 bp hauv 1000 bp.). (2) Lawv tuaj yeem ua kom haum DNA ntau dua li agarose gels.
Vim li cas polyacrylamide siv es tsis txhob agarose thaum ua cov proteins?
Polyacrylamide thiab agarose yog ob qho kev txhawb nqa matrices feem ntau siv hauv electrophoresis. … Agarose muaj qhov pore loj thiab yog haum rau kev sib cais nucleic acids thiab cov protein ntau ntau. Polyacrylamide muaj qhov pore me me thiab zoo tagnrho raucais feem ntau ntawm cov proteins thiab me me nucleic acids.
Vim li cas thiaj siv polyacrylamide gel?
Polyacrylamide gel electrophoresis (PAGE) yog niaj zaus siv rau kev txheeb xyuas cov protein, thiab tseem tuaj yeem siv los cais cov nucleic acid tawg me dua 100 bp. Nucleic acids feem ntau yog tshuaj xyuas siv lub tshuab tsis tu ncua uas muaj qhov tsis sib xws tsis tu ncua, pH, thiab qhov pore loj thoob plaws hauv cov gel.
