2024 Tus sau: Elizabeth Oswald | [email protected]. Kawg hloov kho: 2024-01-13 00:07
Agarose gels siv nrog DNA, vim qhov loj ntawm biomolecules (DNA feem ntau yog ntau txhiab kDa). Rau cov protein gels, polyacrylamide muab daws teeb meem zoo, raws li qhov me me me (50 kDa yog qhov raug) zoo dua rau qhov sib txawv ntawm cov gel.
Qhov txawv ntawm agarose gel electrophoresis thiab polyacrylamide gel electrophoresis yog dab tsi?
Qhov sib txawv tseem ceeb ntawm agarose thiab polyacrylamide yog agarose yog siv rau hauv agarose gel electrophoresis(AGE) feem ntau rau kev sib cais ntawm DNA, hos polyacrylamide yog siv hauv polyacrylamide gel. electrophoresis (PAGE) feem ntau yog rau kev sib cais ntawm cov proteins.
Vim li cas polyacrylamide gels zoo dua agarose gel?
Polyacrylamide gels muaj peb yam tseem ceeb hauv qab no ntawm agarose gels: (1) Lawv qhov kev daws teeb meem zoo heev uas lawv tuaj yeem cais cov molecules ntawm DNA uas nws qhov ntev sib txawv li ntawm 0.1% (piv txwv li, 1 bp hauv 1000 bp.). (2) Lawv tuaj yeem ua kom haum DNA ntau dua li agarose gels.
Vim li cas polyacrylamide siv es tsis txhob agarose thaum ua cov proteins?
Polyacrylamide thiab agarose yog ob qho kev txhawb nqa matrices feem ntau siv hauv electrophoresis. … Agarose muaj qhov pore loj thiab yog haum rau kev sib cais nucleic acids thiab cov protein ntau ntau. Polyacrylamide muaj qhov pore me me thiab zoo tagnrho raucais feem ntau ntawm cov proteins thiab me me nucleic acids.
Vim li cas thiaj siv polyacrylamide gel?
Polyacrylamide gel electrophoresis (PAGE) yog niaj zaus siv rau kev txheeb xyuas cov protein, thiab tseem tuaj yeem siv los cais cov nucleic acid tawg me dua 100 bp. Nucleic acids feem ntau yog tshuaj xyuas siv lub tshuab tsis tu ncua uas muaj qhov tsis sib xws tsis tu ncua, pH, thiab qhov pore loj thoob plaws hauv cov gel.
Pom zoo:
Thaum twg siv kev khav theeb thiab kev khav theeb?
Qhov txawv ntawm Kev Txaus Siab thiab Kev Txaus Siab yog dab tsi? Kev khav theeb hais txog kev txaus siab uas tus neeg tau txais los ntawm ib yam dab tsi. Kev khav theeb, ntawm qhov tod tes, hais txog kev khav theeb. Qhov txawv ntawm ob lo lus no yog thaum kev khav theeb siv tau los ua lub npe lossis lus qhia, txaus siab tsuas yog siv tau ua ib qho adjective.
Tshuaj allopathic yog dab tsi thiab thaum twg yuav tsum tau siv?
Ib kab ke uas cov kws kho mob thiab lwm tus kws kho mob (xws li kws saib xyuas neeg mob, kws muag tshuaj, thiab kws kho mob) kho cov tsos mob thiab kab mob siv tshuaj, hluav taws xob, lossis phais. Kuj hu ua biomedicine, cov tshuaj pa, cov tshuaj tseem ceeb, tshuaj orthodox, thiab Western tshuaj.
Thaum twg siv i.e. thiab thaum twg siv xws li?
I.e. yog ib qho luv luv rau kab lus id est, uas txhais tau tias "uas yog." I.e. yog siv los rov hais dua ib yam dab tsi uas tau hais yav dhau los txhawm rau kom meej nws lub ntsiab lus. E.g. luv luv rau exempli gratia, uas txhais tau tias "
Leej twg pom polyacrylamide gel electrophoresis?
SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis), yog ib qho kev siv hluav taws xob tsis tu ncua tsim los ntawm Ulrich K. Laemmli uas feem ntau siv los ua ib txoj hauv kev los cais cov proteins nrog molecular masses ntawm 5 txog 250 kDa.
Thaum muag ib lub tsev dab tsi yog cov khoom siv thiab khoom siv?
Fixtures yog cov khoom uas txuas nrog rau cov khoom; tsau, yog koj yuav. Fittings yog cov khoom uas tsis txuas nrog cov cuab yeej, tshwj tsis yog los ntawm cov ntiv tes xoos (lossis ntau dua, cov ntsia hlau). Cov khoom siv thiab khoom siv dab tsi suav nrog hauv tsev muag?
