2024 Tus sau: Elizabeth Oswald | [email protected]. Kawg hloov kho: 2024-01-13 00:07
Trypsinization yog cov txheej txheem ntawm kev sib cais ntawm tes siv trypsin, ib qho enzyme proteolytic uas zom cov proteins, kom tshem tawm cov hlwb los ntawm cov hlab ntsha uas lawv tab tom ua. Thaum ntxiv rau kab lis kev cai ntawm tes, trypsin rhuav tshem cov proteins uas ua rau lub hlwb ua raws li lub nkoj.
Vim li cas peb thiaj li sim cov hlwb?
Trypsinization feem ntau ua rau tso cai nkag ntawm cov hlwb mus rau lub thawv tshiab, kev soj ntsuam rau kev sim, lossis txo qis ntawm qhov sib npaug ntawm lub khob los ntawm kev tshem tawm ib feem pua. cov hlwb.
trypsin ua haujlwm li cas kom tshem tawm cov hlwb?
Trypsin / EDTA yog ib txoj hauv kev ua ke rau kev tshem tawm cov hlwb. Trypsin txiav cov adhesion proteins hauv cell-cell thiab cell-matrix kev sib cuam tshuam los ntawm kev txiav cov amino acid ntawm adhesion proteins tshwj xeeb ntawm lysine los yog aginine ntawm C-terminal yog hais tias upstream amino acid tsis proline.
Koj ua li cas thiaj tau trypsinized adherent cells?
txheej txheem
- Tshem tawm nruab nrab ntawm kab lis kev cai nkoj los ntawm kev thov thiab ntxuav lub monolayer nrog ntsev tsis muaj Ca2+ thiab Mg 2+ kom tshem tawm tag nrho cov kab mob ntshav qab zib. …
- Dispense txaus trypsin lossis trypsin / EDTA tov rau hauv kab lis kev cai hlab ntsha kom npog tag nrho cov monolayer ntawm cov hlwb thiab tso rau hauv 37 ° C incubator rau ~ 2 feeb.
Kuv yuav ua li cas lov tes taw trypsin hauv kab lis kev cai ntawm tes?
Ib zaug cov hlwb tshwm sim ntxiv 2 ntimntawm pre-warmed ua tiav kev loj hlob xov xwm kom inactivate trypsin. Maj mam disperse qhov nruab nrab los ntawm pippeting tshaj cell txheej nto ob peb zaug kom paub meej tias rov qab ntawm >95% ntawm cov hlwb. Rau cov kab lis kev cai dawb dawb, Soybean trypsin inhibitor (Cov Khoom No.
Pom zoo:
Wiggling koj txhais taw txhais li cas?
Fidgeting yog ua kom me me nrog koj lub cev, feem ntau yog koj txhais tes thiab ko taw. Nws cuam tshuam nrog kev tsis saib xyuas, thiab feem ntau qhia txog kev tsis xis nyob thiab tsis xis nyob. … Kev ntxhov siab kuj tuaj yeem ua rau tsis xis nyob.
Cov lus txhais txhais li cas hauv Fabkis?
a synonym for [sth] n (lo lus: tib lub ntsiab lus raws li lwm tus) synonyme de [qch] nm + prép. Puas yog lus piv txwv ib yam lossis tawm tsam? ib lo lus muaj tib yam lossis ze li tib yam txhais ua lwm yamhauv lus, zoo siab, xyiv fab, zoo siab.
Potboiler txhais lus txhais lus txhais li cas?
British Dictionary txhais lus rau potboiler potboiler. / (ˈpɒtˌbɔɪlə) / npe. paj ntaub ntawv lossis kos duab ua haujlwm me me ua tau sai sai kom tau nyiaj. lub ntsiab lus ntawm lub lauj kaub yog dab tsi? : a feem ntau ua haujlwm tsis zoo (raws li kev kos duab lossis ntawv nyeem) ua rau muaj txiaj ntsig.
Txhais tes txhais tes txhais li cas?
1a: raug los yog zoo li nrog qee yam taw qhia tshwj xeeb: tsim txom lossis tua los ntawm kev kho ntawm ceg txheem ntseeg ntse. b: txhim kho nyob rau hauv ib qho kev khiav tsis tau lossis tsis muaj kev pab. 2: mus koom (lub tsho tiv no ntawm caj npab) ntawm ib tug heraldic ntaub thaiv npog faib vertically los ntawm daj ntseg.
Txhais lus txhais lus txhais li cas?
rau ncua, tshwj xeeb yog kev khiav tawm lossis kev dag ntxias. … ncua lossis tso tseg, tshwj xeeb tshaj yog los ntawm kev khiav tawm lossis kev dag ntxias (feem ntau tom qab tawm): Nws tau tso tub ceev xwm rau 15 feeb kom nws cov neeg koom tes khiav tawm.
