Hmoob lub nkoj hauv chav sov rau 2-3 feeb . Cov hlwb ua kom ruaj khov tuaj yeem raug tshem tawm sai ntawm 37 ° C. Saib xyuas cov hlwb nyob rau hauv lub microscope. Cov hlwb detached tshwm puag ncig thiab refractile nyob rau hauv lub microscope.
Yuav ua li cas yog tias koj trypsinize hlwb ntev dhau lawm?
Incubating cells nrog siab heev trypsin concentration rau lub sij hawm ntev heev yuav ua rau cell membranes thiab tua cov hlwb. Yog tias tsis paub meej txog qhov concentration ntawm trypsin siv, siv cov concentration tsawg.
Yuav ua li cas koj trypsinize hlwb?
Cells tuaj yeem rov ua dua los ntawm maj mam tso lub xovtooj ntawm kev ncua txhawm rau rhuav tshem cov pawg. Ntxiv dilution tuaj yeem ua tau, yog tias xav tau, rau kev suav ntawm tes thiab / lossis subculturing.
Nws yuav siv sijhawm ntev npaum li cas rau cov cell txuas?
thawj lub hlwb xa mus tom qab 3-5 feeb! Hauv kuv qhov kev paub feem ntau ntawm ECs tau txuas tom qab 10-20min. Tab sis qhov ntev koj tos qhov ruaj khov yog qhov txuas.
Nws puas phem rau Trypsinize hlwb ob hnub ua ke?
Yog nws muaj teeb meem yog tias koj simpsinizing koj cov hlwb tsis tu ncua tom qab 24 teev ntawm kev sib cais. … Koj tuaj yeem tswj xyuas cov hlwb kom txog thaum cov morphology zoo (Nws nyob ntawm koj hom cell, qee lub hlwb tuaj yeem mus txog 80 nqe lus thiab qee qhov mus txog 10).
