Isopycnic gradient centrifugation tshwm sim thaum centrifugation txuas ntxiv mus txog rau thaum tag nrho cov khoom hauv cov gradient tau mus txog qhov chaw uas lawv qhov ntom ntom yog sib npaug ntawm qhov nruab nrab. Hom centrifugation no cais cov khoom sib txawv raws li lawv qhov sib txawv.
Lub hauv paus ntsiab lus ntawm qhov ntom ntom ntom nti centrifugation txheej txheem yog dab tsi?
Density gradient centrifugation tau tshaj tawm tias yog ib qho cuab yeej rau kev sib cais ntawm cov kab mob los ntawm cov khoom noj matrices. Lub hauv paus ntsiab lus yog ua raws li qhov txo qis ntawm kev ncua kev daws teeb meem thiab kev tsiv teb tsaws ntawm cov hom phiaj mus rau qhov sib npaug ntawm cov qauv raj thaum lub sij hawm centrifugation.
Dab tsi yog siv qhov ceev gradient centrifugation?
Density gradient centrifugation enables cov kws tshawb fawb cais cov tshuaj raws li qhov loj me, cov duab, thiab qhov ntom ntom. Meselson thiab Stahl tau tsim ib hom kev ceev gradient centrifugation, hu ua isopycnic centrifugation uas siv cov tshuaj ntawm cesium chloride los cais DNA molecules raws li qhov ceev ib leeg.
Yuav ua li cas yog qhov ceev gradient siv nyob rau hauv sib txawv centrifugation?
Density gradient centrifugation ua kev sib cais ntawm cov khoom kom huv dua li qhov sib txawv ntawm cov centrifugation los ntawm kev siv lub matrix ntom rau cov khoom txav mus los ntawm. Cov txheej txheem sib txawv centrifugation muaj ntau yam centrifugation cov kauj ruam ntawm incrementally nce centrifugalforce.
Dab tsi yog txheej txheem gradient qhov ceev?
Hauv kev tshawb fawb txog lub neej, cov txheej txheem tshwj xeeb hu ua density gradient sib cais yog siv rau kev cais thiab ntxuav cov hlwb, cov kab mob thiab cov kab mob subcellular. Kev hloov pauv ntawm qhov no suav nrog Isopycnic centrifugation, Differential centrifugation, thiab Sucrose gradient centrifugation.