Chromosome conformation capture (3C)-raws li txoj kev nthuav tawm chromosome lub koom haum nyob rau hauv lub nucleus los ntawm kev txiav txim lub cev sib thooj ntawm khub ntawm cov ntsiab lus raws chromatin. Lawv khaws cia chromatin kev sib cuam tshuam los ntawm kev sib txuas sib txuas ua raws li kev sib cais, ligation thiab sib txuas ntawm thaj chaw sib cuam tshuam.
Chromosome conformation capture ua haujlwm li cas?
Chromosome conformation capture-on-chip (4C) captures interactions between one locus and all other genomic loci. Nws koom nrog cov kauj ruam thib ob ligation, los tsim nws tus kheej-circularized DNA tawg, uas yog siv los ua PCR inverse. … Ntawm ib qho microarray, kwv yees li ib lab kev sib cuam tshuam tuaj yeem txheeb xyuas.
Dab tsi yog chromatin conformation capture?
Chromatin Conformation Capture (3C) yog ib qho txheej txheem tseem ceeb siv los kawm chromatin qauv, nrog rau lub hauv paus rau ntau lwm cov txheej txheem derivative. … Cov txheej txheem cuam tshuam nrog formaldehyde hla kev sib txuas ntawm cov hlwb ua raws li kev cais tawm ntawm chromatin thiab zom nrog cov enzyme txwv.
Hi-C sequencing ua haujlwm li cas?
Hi-C mus kom ze txuas ntxiv 3C-Seq rau daim ntawv qhia chromatin hu rau genome-wide, thiab nws kuj tau siv rau kev kawm hauv situ chromatin kev sib cuam tshuam. Hauv txoj kev no, DNA-protein complexes yog crosslinked nrog formaldehyde. Cov qauv yog fragmented, thiab DNA yog muab rho tawm, ligated, thiab zom nrog txwv enzymes.
Hi-C txheej txheem yog dab tsi?
Cov txheej txheem Hi-C classic suav nrog txwv kev zom zaub mov ntawm formaldehyde cross-linked genome nrog cov kab ke tshwj xeeb txwv enzymes, ua raws li kev sau thiab kho cov zom zom xaus nrog kev koom ua ke ntawm biotin txuas nucleotides. Cov kev kho qhov xaus yog tom qab ntawd rov ua dua.
