β-galactosidase yog ib qho enzyme uas hloov galactose mus rau lactose. … Cov txheej txheem coding rau cov enzyme β-galactosidase yog nyiam dua cov tshuaj tiv thaiv kab mob vim tias cov recombinants tuaj yeem pom tau yooj yim thiab cov txheej txheem tsis tshua muaj siab.
Vim li cas cov txheej txheem coding ntawm ib qho enzyme beta-galactosidase yog ib qho kev xaiv xaiv tau zoo dua piv rau cov npe saum toj no?
ntxig rau hauv cov txheej txheem coding ntawm ib qho enzyme, b galactosidase, uas ua rau ua kom cov enzyme hu ua insertional inactivation coding sequence rau cov enzyme b-galactosidase yog nyiam dhau cov tshuaj tiv thaiv kab mob vim tias cov recombinants tuaj yeem. ua tau yooj yim pom.
Yuav ua li cas yog qhov sib lawv liag ntawm β-galactosidase suav tias yog tus cim zoo dua li cov uas tau txheeb xyuas los ntawm koj hauv daim duab?
b Kev nkag ntawm rDNA rau hauvcov txheej txheem coding ntawm ib qho enzyme a-galactosidase ua rau inactivation ntawm enzyme hu ua insertional inactivation. … Yog li coding sequence ntawm a-galactosidase yog tus cim zoo dua.
Tus lej noob rau beta-galactosidase yog dab tsi?
In E. coli, the lacZ gene yog tus qauv gene rau β-galactosidase; uas yog tam sim no ua ib feem ntawm lub inducible system lac operon uas yog activated nyob rau hauv lub xub ntiag ntawm lactose thaum cov piam thaj qis.
Vim li cas nws thiaj xaiv tau tus cim rautshuaj tua kab mob genes?
Cov cim xaiv tau feem ntau yog cov tshuaj tiv thaiv kab mob. Cov no pab hauv cov txheej txheem ntawm kev xaiv khoom. Lawv kuj pab txheeb xyuas cov transformants thiab tso cai xaiv kom loj hlob tab sis kuj tshem tawm cov tsis-transformants.