Dinoflagellate luciferin yog chlorophyll derivative (piv txwv li tetrapyrrole) thiab muaj nyob hauv qee cov dinoflagellates, uas feem ntau yog lub luag haujlwm rau qhov tshwm sim ntawm hmo ntuj glowing nthwv dej (keeb kwm qhov no hu ua phosphorescence., tab sis yog lo lus dag).
Luciferase nyob qhov twg?
Luciferase yog lub teeb tsim cov enzyme ib txwm muaj nyob hauv kab hluav taws thiab hauv cov kab mob hauv hiav txwv thiab cov kab mob hauv av.
tib neeg puas tsim cov luciferin?
Raws li kev tshawb fawb tau ua nyob rau xyoo 2009 los ntawm cov kws tshawb fawb Nyij Pooj, tib neeg bioluminescence nyob rau hauv pom lub teeb muaj tshwm sim- nws tsuas yog dim dhau rau peb lub qhov muag tsis muaj zog tuaj tos. "Lub cev tib neeg lub cev ua kom pom tseeb," pab pawg los ntawm Tohoku Institute of Technology tau sau rau hauv lawv txoj kev tshawb fawb luam tawm hauv PLOS One.
Luciferin siv qhov twg?
Luciferin yog siv, piv txwv li, hauv tus neeg sau xov xwm gene tshuaj ntsuam xyuas los kawm txog kev tswj cov noob thiab kev ua haujlwmqhov kev qhia ntawm tus neeg sau xov xwm luciferin-tagged yog tus cim qhia txog kev ua tiav ntawm cov noob. ntawm kev txaus siab rau cov txheej txheem DNA recombinant.
Koj ua li cas thiaj ua tau luciferin?
Npaj ib 200X luciferin cov tshuaj tov (30 mg / ml) hauv dej tsis muaj menyuam. Sib tov maj mam los ntawm inversion kom txog thaum luciferin tiavyaj. Siv tam sim ntawd, lossis aliquot thiab khov ntawm -20 ° C rau yav tom ntej siv.
