Qhov loj dua qhov hnyav molecular, lub coil ntev dua thiab qeeb cov molecule mus. Yog li, electrophoresis + SDS cais raws li qhov hnyav ntawm molecular, tsis yog raws li tus nqi ib txwm muaj. Cov lus ceeb toom tseem ceeb: Cov protein ntawm tib qhov ntev feem ntau tsis tuaj yeem sib cais los ntawm gel electrophoresis + SDS.
Yuav ua li cas koj cais cov proteins ntawm tib lub cev hnyav?
Centrifugation, electrophoresis, thiab chromatography yog cov txheej txheem ntau tshaj plaws rau kev ntxuav thiab tshuaj xyuas cov protein. Centrifugation cais cov proteins raws li lawv tus nqi sedimentation, uas cuam tshuam los ntawm lawv qhov loj thiab cov duab.
Yuav ua li cas cais cov proteins raws li tus nqi?
Proteins tuaj yeem sib cais raws li lawv cov nqi them los ntawm ion-exchange chromatography. Yog tias cov protein muaj cov txiaj ntsig zoo ntawm pH 7, feem ntau nws yuav khi rau ib kab ntawm cov hlaws dai uas muaj cov carboxylate pawg, qhov uas cov protein tsis zoo yuav tsis (Daim duab 4.4).
Txoj kev twg hauv qab no yog qhov zoo tshaj plaws rau kev cais cov proteins raws li qhov hnyav molecular?
Chromatography cov txheej txheem raws li kev faib tawm tau zoo heev ntawm kev sib cais, thiab kev txheeb xyuas cov molecules me me li amino acids, carbohydrates, thiab fatty acids. Txawm li cas los xij, affinity chromatographies (ie. ion-exchange chromatography) yog qhov zoo dua hauv kev sib cais ntawm macromolecules li nucleic acids, thiab proteins.
Kab hom twgchromatography cais cov proteins raws li qhov hnyav molecular?
Gel filtration (GF) chromatography cais cov proteins nkaus xwb raws li qhov loj ntawm molecular. Kev sib cais yog ua tiav los ntawm kev siv lub tshuab ntxeem tau uas cov molecules, rau qhov tsis zoo, muaj qhov sib txawv ntawm kev nkag tau - piv txwv li, cov khoom me me muaj kev nkag tau ntau dua thiab cov molecules loj dua raug cais tawm ntawm matrix.
