Feem ntau cov buffers thiab lwm cov tshuaj ntsev yog autoclaved, vim tias pom cov ntim loj yog siv sijhawm ntev thiab cov lim tsis huv pov tseg yog kim. Txawm li cas los xij, ua ntej autoclaving ib qho kev daws teeb meem koj yuav tsum nco ntsoov xyuas seb nws puas muaj cov khoom xyaw cua sov-labile.
Tus tsis tuaj yeem ua autoclaved?
Txhawm rau tshem tawm cov kab mob microbial thiab lwm yam kab mob, buffers raug tshem tawm tom qab kev npaj. Txawm li cas los xij, qee qhov buffers tsis yog kub resistant xws li MOPS, HEPES thiab lwm yam. Yog li ntawd, autoclaved dej yuav siv tau rau kev npaj buffers.
Yuav siv tshuaj dab tsi hauv autoclave?
Kev ua haujlwm zoo yog qhov tseem ceeb rau txoj kev ua kom tsis muaj menyuam autoclave. Cov chav uas siv los ua kom tsis muaj menyuam yuav tsum yog 97% chav (vapor) thiab 3% noo noo (kua dej). Qhov piv txwv no tau pom zoo rau qhov hloov pauv tau zoo tshaj plaws.
Dab tsi ua kua tsis tuaj yeem ua autoclaved?
Cov Khoom Siv Tsis Tau Rau Autoclaving
Raws li txoj cai dav dav ntawm tus ntiv tes xoo, koj TSIS TAU cov ntaub ntawv autoclave uas muaj kab mob cov kuab tshuaj, cov khoom siv hluav taws xob, cov tshuaj tsis haum lossis cov tshuaj corrosive, lossis cov khoom uas muaj mutagens, carcinogens, los yog teratogens.
Yuav ua li cas kom tsis muaj menyuam?
Sterilization yog pom zoo rau feem ntau cov ntawv thov thiab feem ntau ua tiav los ntawm autoclaving. Cov khoom uas muaj cov khoom uas tsis hloov pauv, hloov pauv lossis puas los ntawm cov cua sov, lossis nws cov pH lossis concentration yog qhov tseem ceebsterilized los ntawm kev pom los ntawm 0.22-µm lim.