DNase Kuv qhov chaw puas siab puas ntsws pom qhov twg thiab lawv qhia dab tsi txog qhov xwm txheej ntawm chromatin? Cov chaw no muaj nyob ntawm exon-intron junctions thiab qhia qhov chaw ntawm mRNA cleavage thaum lub sij hawm splicing. Cov chaw no raug random pom thoob plaws hauv genome thiab nws ua rau DNA yooj yim rau degradation.
DNase kuv qhov chaw hypersensitive yog dab tsi thiab nws cuam tshuam li cas chromatin?
Nyob hauv noob caj noob ces, DNase I hypersensitive sites (DHSs) yog cov cheeb tsam ntawm chromatin uas nkag siab rau kev sib cais los ntawm DNase I enzyme. Hauv cov cheeb tsam tshwj xeeb ntawm genome, chromatin tau poob nws cov qauv condensed, nthuav tawm DNA thiab ua rau nws nkag mus tau.
DNase hypersensitive site qhia dab tsi?
Cov ntsiab lus cis-kev tswj hwm yog qhov ua rau muaj kev cuam tshuam los ntawm endonuclease DNase I. Motifs nyob rau hauv DNase I hypersensitive sites qhia muaj peev xwm hloov pauv qhov ua haujlwm nyob thiab, thaum ua ke nrog DNase I cleavage cov ntaub ntawv, tuaj yeem siv los tsim cov kev tswj xyuas ib ntus.
DNase kev ntsuas ntshav siab yog dab tsi?
Rau ntau tshaj li ob xyoo lawm, DNase I hypersensitivity assay (DHA) tau instrumental rau nrhiav pom cov cai tswj hwm(11). Txoj kev no qhia cov chaw sib cais hauv cov genome uas qhov sib haum ntawm chromatin ua rau nws cov DNA hypersensitive rau cleavage los ntawm endonuclease DNase I (12).
Nuclease hypersensitive site hauv chromatin yog dab tsi?
Ibchromatin, nucleosome-free regions hu ua nuclease hypersensitive sites ntseeg tau tias sawv cev rau "qhib qhov rais" uas tso cai rau kev nkag mus tau zoo ntawm cov neeg nyob hauv cis-acting DNA ib ntus rau cov yam ntxwv hloov pauv(saib Refs.
