Qhov sib piv, feem ntau eukaryotic DNA ligases, ua ke nrog archaeal thiab bacteriophage enzymes, poob rau hauv tsev neeg thib ob; Cov enzymes no siv ATP ua cofactor.
Pob ligases xav tau ATP?
Ob DNA ligases feem ntau siv. … T4 ligase xav tau ATP thaum E. coli ligase xav tau NAD+ (Nicotinamide adenine dinucleotide yog coenzyme uas muaj 2 nt koom los ntawm lawv pawg phosphate.). Ob qho tib si catalyze kev koom tes ntawm 5'-phosphate thiab 3'-OH pawg los tsim ib daim ntawv cog lus phosphodiester.
Vim li cas ligases xav tau ATP?
Ob kauj ruam ntawm DNA ligation cov tshuaj tiv thaiv
Cov tshuaj tiv thaiv DNA ligation nws tus kheej muaj ob kauj ruam yooj yim. Ua ntej cov DNA kawg yuav tsum sib tsoo los ntawm lub sijhawm thiab nyob ua ke ntev txaus rau cov ligase koom nrog lawv. … Txhawm rau tso cai rau cov enzyme ua cov tshuaj tiv thaiv ntxiv rau AMP hauv qhov chaw ua haujlwm enzyme yuav tsum tau ntxiv los ntawm ATP.
Yuav ua li cas yog ligases?
DNA ligases ua lub luag haujlwm tseem ceeb hauv tuav txoj kev ncaj ncees ntawm genomic los ntawm kev koom nrog kev so hauv phosphodiester qaum ntawm DNA uas tshwm sim thaum rov ua dua thiab rov ua dua, thiab ua rau muaj txiaj ntsig ntawm DNA puas thiab nws kho. Peb tib neeg cov noob, LIG1, LIG3 thiab LIG4 encode ATP-dependent DNA ligases.
Dab tsi yog ligases lawv ua haujlwm nquag lossis ua ntu zus?
DNA ligase yog nquag thaum lub sijhawm rov ua dua, kho thiab rov ua dua txheej txheem ntawm DNA. Nws yog dav siv nyob rau hauv kev kho ntawm ib leeg-strandtawg nyob rau hauv duplex DNA ntawm cov kab mob nyob uas siv lub complementary strand ntawm ob helix raws li ib tug template, thaum ib co ntaub ntawv tej zaum tshwj xeeb kho ob-strand puas.
