Yes tseeb, yog tias chloroform tsis raug tshem tawm ntawm phage npaj. Chloroform feem ntau yog ntxiv rau phage cais / ntxuav cov kauj ruam kom tshem tau cov kab mob. … Chloroform tsis yog ib kauj ruam tseem ceeb thiab yuav tsum tau coj mus rau hauv tus account tias nws yuav ua rau lwm cov phages muaj nyob hauv cov kab mob.
chloroform ua dab tsi rau E coli?
Chloroform hauv cov ntsiab lus no tua cov kab mob sai. Koj tuaj yeem ua pov thawj los ntawm titering rau CFU ua ntej thiab tom qab co nrog CHCl3. Yuav kom tau txais phage tshiab los ntawm cov kab mob induced yuav siv sij hawm ib pliag (lub sij hawm latent los ntawm induction) thiab tsis zoo li los ntawm cov kab mob sai sai.
Lub luag haujlwm ntawm chloroform yog dab tsi hauv kev cais cov kab mob bacteriophage?
Nrog cov coli-phages chloroform kev kho mob feem ntau yog siv los tua cov kab mob tam sim no thiab cov kauj ruam lim-sterilization yog qee zaum tshem tawm. Chloroform tuaj yeem cuam tshuam qee qhov phages thiab yuav tsum tau siv nrog ceev faj thiab tswj kom raug. Sodium azide kuj tseem siv tau rau qee qhov phages.
Kev siv chloroform yog dab tsi los npaj lysate thaum phage cais?
Cov tshuaj chloroform tau ntxiv rau 15-20 feeb kom lyse cov hlwb thiaj li tso tawm cov kab mob mus rau nruab nrab.
Koj cais cov kab mob bacteriophages li cas?
Kev cais ntawm cov kab mob bacteriophages rau phage kho feem ntau nthuav tawm raws li qhov ncaj ncaj kev tawm dag zog ntawm kev sib xyaw phage-muaj cov qauv nrog cov kab mob hauv tsev, ua raws li kev tshem tawm cov kab mob yooj yim.los ntawm kev pom thiab / lossis centrifugation hnub tom qab [1, 2, 3].
