Kev tshuaj ntsuam ob-hybrid yog cov txheej txheem biology molecular siv los tshawb pom cov protein-protein kev sib cuam tshuam thiab protein-DNA kev sib cuam tshuam los ntawm kev sim rau kev sib cuam tshuam ntawm lub cev ntawm ob cov proteins lossis ib qho protein thiab DNA molecule, feem.
Yeast ob-hybrid mus kom ze yog dab tsi?
Cov poov xab ob-hybrid yog raws li kev hloov kho ntawm qhov kev hloov pauv hloov pauv (TF) thaum ob lub proteins lossis polypeptides ntawm kev txaus siab cuam tshuam. … Raws li kev sib cuam tshuam ntawm cov kab nuv ntses thiab cov tsiaj nyeg, DBD thiab AD raug coj los nyob ze thiab ua haujlwm TF tau rov tsim dua los ntawm cov neeg sau xov xwm gene.
Yeast ob-hybrid siv rau dab tsi?
Cov poov xab ob-hybrid (Y2H) assay yog ib lub cuab yeej muaj zog los txheeb xyuas binary PPIs [6] los ntawm kev siv cov qauv ntawm cov poov xab Gal4 transcription factor. Hauv qhov kev ntsuam xyuas no, DNA-binding domain thiab activation domain ntawm Gal4 yog fused rau ob proteins txaus siab.
kab mob ob-hybrid yog dab tsi?
Cov kab mob ob-hybrid (BACTH, rau "Bacterial Adenylate Cyclase-Based Ob-Hybrid") system yog ib txoj hauv kev yooj yim thiab ceev caj ces txhawm rau txhawm rau txheeb xyuas thiab ua kom muaj kev sib cuam tshuam ntawm cov protein-protein hauv vivo. … Tsis tas li ntawd, cov proteins ntawm cov kab mob tuaj yeem kawm tau nyob rau hauv ib puag ncig zoo sib xws (lossis zoo ib yam) rau lawv haiv neeg.
Puas yog 2 lub hybrid?
Qhov ob-hybrid system yog kev tshuaj ntsuam genetic poov xab rau kev kuaj xyuas cov protein-protein sib cuam tshuam. Nws tuaj yeem yogsiv los txheeb xyuas cov proteins uas khi rau cov protein uas txaus siab, lossis txiav txim siab cov npe lossis cov seem tseem ceeb rau kev sib cuam tshuam.
