Vim DdNTPs muaj hydrogen molecule (-H) es tsis txhob ntawm ib pawg hydroxyl (-OH) txuas rau 3'-C ntawm nws cov deoxyribose, nws tsis tuaj yeem khi rau ib qho nucleotides tuaj. Yog li ntawd, ntxiv ntawm DdNTPs thaum lub sij hawm DNA replication tuaj yeem siv los txiav tawm cov tshuaj tiv thaiv synthesis.
Vim li cas DNA synthesis nres thaum ddNTP tau koom ua ke rau hauv cov DNA loj hlob?
Yog cov nucleotide ntxiv yog "dideoxynucleotide" (Daim duab 6.29), 3' kawg ntawm loj hlob strand tam sim no yuav muaj H, tsis yog OH. Qhov no yuav tiv thaiv cov nucleotides ntxiv los ntawm kev ntxiv rau; piv txwv li, hauv vitro DNA synthesis yuav nres ntawm qhov no.
DdNTP ua li cas thiaj txiav DNA synthesis?
Thaum ib qho ddNTP tau koom ua ke rau hauv cov saw ntawm nucleotides, kev sib txuas yuav xaus. Qhov no yog vim hais tias ddNTP molecule tsis muaj 3 'hydroxyl pawg, uas yuav tsum tau tsim ib qho txuas nrog cov nucleotide tom ntej hauv cov saw hlau.
Vim li cas DdNTPs xaus cov txheej txheem rov ua dua?
Kev Tshawb Fawb Txoj Kev hauv Enzymology: DNA
Dideoxynucleotide triphosphates tau nkag mus rau hauv cov saw DNA loj hlob, tab sis tsis muaj 3′ hydroxyl pab pawg tsim nyog tso cai rau cov saw txuas ntxiv, thiab tshem tawm cov polymerization zoo.
Vim li cas Dideoxynucleotides ua rau DNA rov ua dua?
Cov ddNTPs tsis muaj 3′-OH pawg uas yuav tsum tau tsim kom muaj phosphodiester daim ntawv cog lus ntawm obnucleotides, ua rau qhov txuas ntxiv ntawm DNA strand nres thaum ntxiv ddNTP.
