2024 Tus sau: Elizabeth Oswald | [email protected]. Kawg hloov kho: 2024-01-13 00:07
Txawm li cas los xij, thaum emission spectra overlap, fluorescence los ntawm ntau tshaj ib qho fluorochrome yuav raug kuaj pom. Txhawm rau kho qhov kev sib tshooj spectral no, siv cov txheej txheem ntawm kev them nyiaj fluorescence. Qhov no ua kom ntseeg tau tias cov fluorescence pom nyob rau hauv ib qho kev ntes tau los ntawm fluorochrome uas tau ntsuas.
Vim li cas peb thiaj li xav tau kev them nyiaj hauv kev ntws cytometry?
Kev them nyiaj yuav tsum tau ua rau kev sim cytometry ntws vim lub physics ntawm fluorescence. Lub fluorochrome zoo siab heev, thiab emit ib tug photon nyob rau hauv ib tug ntau yam ntawm wavelengths. Qee qhov ntawm cov photons nchuav mus rau hauv qhov ntsuas thib ob, ua rau ib qho stained kuaj pom ob qhov zoo.
Lub hom phiaj tseem ceeb ntawm kev siv lub xov tooj them nyiaj yog dab tsi?
Lub hom phiaj tseem ceeb yog tso cai ntsuas qhov tseeb fluorescence hauv thawj cov channel uas kis tau los ntawm cov fluorophores nyob sib ze. Yog li, kev them nyiaj kho rau fluorescence "trespass" ntawm fluorophores. Txawm li cas los xij, nws tsis zoo tag nrho thiab kho tsis tau tag nrho cov txiaj ntsig tsis zoo.
nyiaj them matrix yog dab tsi?
Ib qho kev them nyiaj matrix yog xam siv cov xim fluorescent tswj cov ntaub ntawv uas tau sau rau ntawm ImageStream lossis FlowSight nrog txhua txoj kev sau thiab thaum tsis muaj lub teeb ci ntsa iab lossis SSC.
Kev them nyiaj spectral yog dab tsi?
Siv spectralkev them nyiaj txhais tau hais tias lub cim los ntawm cov cuab yeej tsis yog lub hauv paus tseem siv los txhim kho cov teeb liab tseeb ntawm cov cim qhov ua tau. Cov qauv tswj thawj yog siv rau parameter npe. … Cov qauv siv rau cov cuab yeej tseem ceeb kuj tseem pab sau cov npe ntawm cov tsis muaj tom qab them nyiaj spectral.
Pom zoo:
Vim li cas cov nyiaj khwv tau los tsis tau them se?
teb yooj yim – lawv tsis ua! Tsis zoo li cov txiaj ntsig tau txais uas tau them se txhua zaus koj muag; Cov nyiaj tsis tau them se tsis tau them se vim lawv yog cov txiaj ntsig uas koj tsuas pom hauv daim ntawv thiab tsis muaj dab tsi tau ua tiav.
Vim li cas kuv yuav tsum khaws kuv daim ntawv them nyiaj?
Ob tus tswv ntiav thiab cov neeg ua haujlwm yuav tsum khaws lawv daim ntawv them nyiaj. Daim ntawv them nyiaj muaj ntau yam ntawm cov se tseem ceeb thiab cov ntaub ntawv nyiaj txiag. Rau cov neeg ua haujlwm, cov ntaub ntawv no tuaj yeem siv los pab lawv txheeb xyuas lawv cov nyiaj tau los, them se, thiab xyuas kom lawv tau txais kev them nyiaj ncaj ncees rau lawv txoj haujlwm.
Cov nyiaj them rov qab puas yuav tsum tau them nqi?
Raws li tus tswv lag luam me lossis tus neeg ua haujlwm ywj pheej, nws tsis yog qhov tsis yooj yim uas yuav tau them nuj nqis rau koj cov neeg siv khoom thaum ua haujlwm ntawm cov haujlwm. Cov no yog lub npe hu ua cov nuj nqis them rov qab, thiab nws tau zoo kawg nkaus los them koj cov neeg siv khoom rau lawv.
Kuv puas yuav raug them rau qhov tsis tau them nyiaj tshaj tawm santander?
Yog tias peb tso cai them nyiaj, nws yuav coj koj mus rau qhov tsis tau them nqi ntxiv. Peb tsis them nqi rau kev tso cai lossis tsis kam them vim tsis muaj nyiaj. Yuav ua li cas yog tias koj mus rau hauv qhov nyiaj tsis tau them nqi?
Yuav tsum them nyiaj severance los ntawm kev them nyiaj?
Lub Tebchaws Meskas Lub Tsev Hais Plaub Siab tau txiav txim siab tias cov nyiaj them poob haujlwm yog tiag tiag cov nyiaj ua haujlwm tsis tu ncua uas yuav raug them se ua ntu zusthem se. … Cov tswv hauj lwm yuav tsum tau txiav 22% ntawm cov nyiaj them poob haujlwm thiab them nyiaj rau IRS.
