2024 Tus sau: Elizabeth Oswald | [email protected]. Kawg hloov kho: 2024-01-13 00:07
Vim sonication tuaj yeem muaj peev xwm txo qis cov proteins thiab denature protein epitopes, peb tom ntej ntsuas qhov cuam tshuam ntawm sonication ntawm kev ncaj ncees ntawm cov protein.
Sonication puas muaj protein ntau?
Cov Lus Teb Nrov (1)
Standard sonication raws tu qauv rather tsis tuaj yeem ua rau muaj protein ntau fragmentation- lub zog tsawg dhau. Nws yuav tsum tsis txhob ua rau nws denaturation. Nws tuaj yeem ua denaturated thaum koj sonicate nws ntev dhau lawm thiab overheat tus qauv. Yog tias koj siv nickel chromatrograpy lwm cov bands tej zaum yuav tsuas yog impurities.
Sonication puas tawg peptide bonds?
Sonication ib txwm tsis tuaj yeem tawg ib qho protein cov qauv vim tias cov qauv (cov amino acid sequence) yog tsim los ntawm covalent bonds uas muaj ntau zaus ntau dua li Hydrogen bonds thiab sonication tau muab lub zog xav tau los rhuav tshem cov ntawv cog lus.
Puas sonication tawg nuclear membrane?
A luv sonication nrog lub zog qis tuaj yeem cuam tshuamcov ntshav plasma tsis tawg ntau mitochondrial lossis nuclear membranes yielding hauv tact mitochondria.
Puas sonication tsim nyog rau kev rho tawm cov protein?
Detergent solubilization (piv txwv li, SDS) uas yuav tsum tau rau kev sib cais ntawm cov kua nplaum uas ua rau cov kua nplaum zoo li lysate feem ntau vim yog nucleic acids. Sonication yog li ntawd yuav tsum tau nyob rau hauv xws li mob los cuam tshuam cov nucleic acids. Sonication ua kom sov ntauthiab tsis zoo rauprotein ntau.
Pom zoo:
Koj puas tuaj yeem tau txais cov ntawv rhuav tshem tus tswv?
Master spells tsis tuaj yeem yuav pib. Txhawm rau qhib Master Destruction spells, quest "Kev puas tsuaj Ritual Spell" yuav tsum ua kom tiav thaum ncav cuag qib 100 Kev puas tsuaj. Kuv yuav ua li cas thiaj li tau txais tus tswv kev puas tsuaj?
Mcd puas tuaj yeem rhuav tshem yam tsis tau ceeb toom?
Kev txiav txim rhuav tshem tsis yog txhaum cai tab sis tsis raug cai ua kev rhuav tshem yam tsis tau tshaj tawm tsab ntawv ceeb toom rau tus neeg nyob, … Xa daim ntawv foob rau kev rhuav tshem tau ua txhaum cai yam tsis ua haujlwm ceeb toom rau koj.
Pepsin puas tuaj yeem rhuav tshem cov piam thaj?
Y Protein digestion tshwm sim hauv plab thiab duodenum los ntawm kev ua ntawm peb lub ntsiab enzymes: pepsin, secreted los ntawm lub plab, thiab trypsin thiab chymotrypsin, secreted los ntawm lub txiav. Thaum lub sijhawm kev zom zaub mov carbohydrate, kev sib txuas ntawm cov piam thaj molecules raug rhuav tshem los ntawm qaub ncaug thiab pancreatic amylase.
Koj puas tuaj yeem rhuav tshem tus menyuam?
Cov niam txiv puas tuaj yeem rhuav tshem peb cov menyuam? Nws yog qhov ua tau, hais tias Dr. Michele Borba, tus kws tshaj lij kev xav txog kev kawm thiab tus kws sau ntawv zoo tshaj plaws ntawm "UnSelfie: Vim Li Cas Cov Menyuam Uas Muaj Kev Txaus Siab Hauv Peb Lub Ntiaj Teb Txhua Yam Txog Kuv.
Koj puas tuaj yeem rhuav tshem cov lus cog tseg rau hnub Sunday?
Tseem, thaum peb muab ib yam dab tsi rau qiv, qhov ntawd yog ib qho kev yoo mov. Yog li ntawd, kev txi tsis raug khi rau Hnub Sunday hauv Qiv, vim tias, zoo li txhua txhua hnub Sunday, Hnub Caiv yog ib txwm noj mov. Hnub Sunday yog hnub khib nyiab thaum qiv?
