Vim sonication tuaj yeem muaj peev xwm txo qis cov proteins thiab denature protein epitopes, peb tom ntej ntsuas qhov cuam tshuam ntawm sonication ntawm kev ncaj ncees ntawm cov protein.
Sonication puas muaj protein ntau?
Cov Lus Teb Nrov (1)
Standard sonication raws tu qauv rather tsis tuaj yeem ua rau muaj protein ntau fragmentation- lub zog tsawg dhau. Nws yuav tsum tsis txhob ua rau nws denaturation. Nws tuaj yeem ua denaturated thaum koj sonicate nws ntev dhau lawm thiab overheat tus qauv. Yog tias koj siv nickel chromatrograpy lwm cov bands tej zaum yuav tsuas yog impurities.
Sonication puas tawg peptide bonds?
Sonication ib txwm tsis tuaj yeem tawg ib qho protein cov qauv vim tias cov qauv (cov amino acid sequence) yog tsim los ntawm covalent bonds uas muaj ntau zaus ntau dua li Hydrogen bonds thiab sonication tau muab lub zog xav tau los rhuav tshem cov ntawv cog lus.
Puas sonication tawg nuclear membrane?
A luv sonication nrog lub zog qis tuaj yeem cuam tshuamcov ntshav plasma tsis tawg ntau mitochondrial lossis nuclear membranes yielding hauv tact mitochondria.
Puas sonication tsim nyog rau kev rho tawm cov protein?
Detergent solubilization (piv txwv li, SDS) uas yuav tsum tau rau kev sib cais ntawm cov kua nplaum uas ua rau cov kua nplaum zoo li lysate feem ntau vim yog nucleic acids. Sonication yog li ntawd yuav tsum tau nyob rau hauv xws li mob los cuam tshuam cov nucleic acids. Sonication ua kom sov ntauthiab tsis zoo rauprotein ntau.
