2024 Tus sau: Elizabeth Oswald | [email protected]. Kawg hloov kho: 2024-01-13 00:07
Chromatography nyob rau hauv nws ntau hom yog kab tias txoj kev paub tseem ceeb tshaj plaws rau kev tshuaj xyuas tshuaj ntawm cov khoom sib tov. Daim ntawv thiab nyias-txheej chromatography yog cov txheej txheem yooj yim uas siv tau cais cov sib tov rau hauv ib tus neeg cov khoom.
Txoj kev twg yog siv los cais cov sib xyaw ua ke?
Centrifugation cais cov sib tov sib xyaw ua ke los ntawm kev tig lawv ntawm qhov nrawm heev, uas yuam cov khoom sib cais rau hauv txheej.
Yuav ua li cas sib tov yog chromatography siv los cais?
Chromatography yog ib txoj hauv kev los cais cov khoom ntawm ib homogeneous sib xyawraws li qhov sib txawv ntawm cov khoom sib txawv hauv cov kuab tshuaj lossis cov kuab tshuaj sib tov. Feem ntau nws tau ua hauv cov kua lossis hauv cov pa roj.
Yuav ua li cas sib xyaw heterogenous sib cais?
Ib qho sib xyaw ua ke yog kev sib xyaw ntawm ob lossis ntau yam tshuaj lom neeg (cov ntsiab lus lossis cov khoom sib txuas), qhov sib txawv ntawm cov khoom tuaj yeem pom qhov txawv thiab yooj yim cais los ntawm lub cev txhais tau tias.
Qhov twg Tsis tuaj yeem siv los cais cov sib xyaw ua ke?
Centrifugation: Qee zaum cov khoom hauv cov kua muaj me me thiab tuaj yeem dhau los ntawm daim ntawv lim. Rau cov khoom zoo li no, cov txheej txheem pom tsis tuaj yeem siv rau kev sib cais. Xws li cov khoom sib xyaw yog sib cais los ntawm centrifugation.
Pom zoo:
Puas yog cov roj chlorine yog cov khoom lossis cov khoom sib xyaw?
Chlorine yog nyob rau hauv pawg 17 ntawm lub sij hawm lub sij hawm, tseem hu ua lub halogens, thiab tsis pom raws li lub caij nyob rau hauv cov xwm - tsuas yog ib tug compound. Feem ntau ntawm cov no yog ntsev, los yog sodium chloride, thiab cov poov tshuaj compounds sylvite (los yog potassium chloride) thiab carnallite (potassium magnesium chloride hexahydrate).
Kev sib xyaw ua ke puas tuaj yeem sib cais los ntawm lub cev txhais tau tias?
Kev sib xyaw tuaj yeem sib cais los ntawm lub cev txhais tau tias Txhua qhov sib xyaw , txawm tias homogeneous lossis heterogeneous, sib koom ib qho khoom sib xws: Lawv tuaj yeem muab cais ua ntau hom teeb meem hom teeb meem hauv physics, ib lub xeev ntawm teeb meem yog ib qho ntawm cov ntaub ntawv uas muaj teeb meem.
Puas yog kev sib tw tsis sib xws tib yam li kev sib xyaw ua ke?
Kev txwv tsis pub sib tw yog qee zaum xav tias yog tshwj xeeb ntawm kev sib xyaw inhibition. Hauv kev sib xyaw inhibition, tus inhibitor khi rau ib qho chaw allosteric, piv txwv li ib qhov chaw sib txawv ntawm qhov chaw nquag uas lub substrate khi.
Puas cov sib tov sib xyaw ua ke muaj cov khoom sib xws?
Ib homogeneous sib tov yog sib tov nyob rau hauv uas muaj pes tsawg leeg yog uniform thoob plaws qhov sib tov .. Qee cov ntaub ntawv hais ntxiv tias cov tshuaj lom neeg tsis tuaj yeem muab cais rau hauv nws cov ntsiab lus los ntawm kev sib cais ntawm lub cev, piv txwv li, tsis muaj kev cuam tshuam tshuaj lom neeg.
Thaum twg yog chromatography cov txheej txheem sib cais zoo tshaj plaws?
Cov txheej txheem chromatography raws li kev faib tawm tau zoo heev ntawm kev sib cais, thiab txheeb xyuas cov molecules me me li amino acids, carbohydrates, thiab fatty acids. Txawm li cas los xij, affinity chromatographies (ie. ion-exchange chromatography) muaj txiaj ntsig zoo dua hauv kev sib cais ntawm macromolecules li nucleic acids, thiab proteins.
