Rau qhov hloov pauv-theem chromatography yam yog, zoo, qhov thim rov qab. Koj siv cov theem tsis yog-polar nyob ruaj ruaj uas khaws cov tsis-polar tebchaw thiab yog li, koj elute ua ntej lub polar molecules polar molecules Hauv chemistry, polarity yog kev sib cais ntawm cov nqi hluav taws xob ua rau cov molecule lossis nws cov tshuaj pawg muaj lub sijhawm hluav taws xob dipole, nrog qhov tsis zoo them kawg thiab qhov kawg them zoo. Polar molecules yuav tsum muaj polar bonds vim qhov sib txawv ntawm electronegativity ntawm cov atoms sib txuas. https://en.wikipedia.org › wiki › Chemical_polarity
Tshuaj polarity - Wikipedia
Koj ua li cas txiav txim siab seb qhov twg yuav ua rau thawj zaug hauv kab chromatography?
Ib tug tsawg-polar solvent yog thawj zaug siv los elute ib tug tsawg-polar compound. Thaum qhov tsawg-polar compound tawm ntawm kem, cov kuab tshuaj ntau dua-polar ntxiv rau hauv kem kom elute qhov ntau-polar compound.
Yuav ua li cas nrawm dua hauv kab chromatography?
Hauv kab chromatography, cov molecules reversibly adsorb mus rau theem nyob ruaj khov thaum lawv ntws los ntawm kem, yog li ua rau lawv txoj kev vam meej. Cov tshuaj uas cuam tshuam tsis muaj zog nrog rau theem nyob ruaj khov nrawm dua kom tawm ntawm kab, lossis hais lus. Cov tebchaw uas cuam tshuam nrog cov theem nyob ruaj khov yog qeeb qeeb rau elute.
Yam kev elution yog dab tsi?
Elution feem ntau follows lub boilcov ntsiab lus ntawm cov compounds.
Yuav ua li cas thiaj li yuav tsum cov khoom sib txuas tawm ntawm kab?
- Nyob rau hauv ib kem ib txwm, cov theem nyob ruaj khov yog ntau dua polar dua li theem mobile. …
- Nyob rau hauv ib kem ib txwm, peb lub tebchaw tau eluted nyob rau hauv raws li nram no: p-dimethylbenzene, p-dimethoxybenzene, ces p-methoxyphenol.
