Thaum lub sijhawm DNA rov ua dua (luam tawm), feem ntau DNA polymerases tuaj yeem "kuaj lawv cov haujlwm" nrog txhua lub hauv paus uas lawv ntxiv. Cov txheej txheem no hu ua proofreading. … Polymerase ntaus tias cov hauv paus yog mispaired. Polymerase siv 3' mus rau 5' exonuclease kev ua kom tshem tawm qhov tsis raug T los ntawm 3' kawg ntawm txoj hlua tshiab.
Kev ua pov thawj ua dab tsi hauv DNA?
DNA polymerase proofreading yog ib qho kev tshuaj xyuas kev sau ntawv uas ua kom DNA polymerases tshem tawm cov kev tsim tshiab nucleotide incorporation yuam kev los ntawm primer terminus ua ntej ntxiv primer txuas ntxiv thiab tseem tiv thaiv kev hloov pauv.
DNA polymerase twg ua pov thawj hauv DNA replication?
Ib txoj haujlwm tseem ceeb ntawm kev rov ua dua DNA polymerases yog rov ua dua DNA nrog qhov raug siab heev. Kev ncaj ncees ntawm DNA replication relies on nucleotide selectivity ntawm replicative DNA polymerase, exonucleolytic proofreading, thiab postreplicative DNA mismatch kho (MMR).
Kev nyeem ntawv pov thawj ua li cas rau qhov raug ntawm DNA rov ua dua?
Kev Tshawb Fawb los ntawm DNA polymerase kho qhov yuam kev thaum rov ua dua. Qee qhov yuam kev tsis raug kho thaum rov ua dua, tab sis hloov kho tom qab rov ua tiav; Hom kev kho no yog hu ua kev kho tsis sib haum (Daim duab 2). … Hauv kev kho tsis sib haum, qhov tsis raug ntxiv hauv paus raug kuaj pom tom qab rov ua dua.
Qhov kev ua enzyme twg koom nrogproofreading thaum DNA replication?
Lwm qhov tseem ceeb ntawm lub luag haujlwm rau qhov raug ntawm DNA replication yog qhov kev ua pov thawj ntawm DNA polymerase. Raws li twb tau sau tseg lawm, E. coli polymerase kuv muaj 3′ txog 5′ as well as 5′ rau 3′ exonuclease act.
