Cov kws tshawb fawb feem ntau siv Trypan Blue rau ob qho tib si TNC thiab cell viability suav. Txawm li cas los xij rau cov qauv uas muaj cov qe ntshav liab (RBCs), feem ntau nyuaj rau kev paub qhov txawv cov hlwb nucleated los ntawm RBCs siv Trypan Blue ib leeg.
Puas trypan xiav stain nyob hlwb?
Trypan xiav tau ntev dhau los ua tus qauv kub rau staining cell tuag los txiav txim siab cell viability. Lub dye yog cais tawm ntawm cov cell uas tsis muaj nyob hauv daim nyias nyias, tab sis tuaj yeem nkag mus thiab mloog zoo rau hauv cov cell uas muaj kab mob sib kis, ua rau cov hlwb xiav tsaus.
trypan xiav stain yog dab tsi?
Trypan xiav yog lub xov tooj ntawm tes tsis raug siv los kwv yees tus lej ntawm cov hlwb tuag hauv cov neeg siv tau. Nws qhov kev siv hluav taws xob yog raws li qhov tseeb tias nws yog cov xim dye thiab tsis nkag mus rau hauv cov cell tshwj tsis yog tias daim nyias nyias raug cuam tshuam.
cov qe ntshav liab stained nrog dab tsi?
Ntawm no yog daim duab tsis muaj zog ntawm cov ntshav smear stained May Grunwald Giemsa stain. Qhov no stains acidic proteins nyob rau hauv erythrocytes liab, raws li hemoglobin yog eosinophilic (acidophilic). Tag nrho cov hlwb hauv daim duab no yog cov qe ntshav liab, qhia tias lawv muaj ntau npaum li cas!
Cov qe ntshav liab tshwj xeeb li cas?
cov qe ntshav liab ua tsis muaj nuclei, tso cai rau ntau chav rau hemoglobin. Cov duab ntawm cov qe ntshav liab yog ib qho tshwj xeeb biconcave zoo li (puag ncig nrog lub tiaj, indented center). Lawv qhov tsis muaj nuclei ua rau lawv hloov tau li ntawdlawv tuaj yeem hla cov hlab ntsha me me.
