pBR322 yog 4361 lub hauv paus khub ntev thiab muaj ob hom tshuaj tiv thaiv kab mob - cov noob Bla encoding cov tshuaj ampicillin tsis kam (AmpR) protein, thiab gene tetA encoding cov tetracycline tsis kam (TetR) protein.
Ntawm qhov chaw ntawm pBR322 cov tetracycline resistant noob nyob qhov twg?
- BamH 1 site rau kev txwv enzyme muaj nyob rau ntawm cov noob rau tetracycline tsis kam.
Txoj kev txwv enzyme dab tsi koj yuav siv los txiav cov tetracycline tsis kam gene los ntawm pBR322?
pBR322 muaj cov noob rau tetracycline thiab ampicillin tsis kam enzymes, nrog rau cov chaw uas nws tuaj yeem raug txiav los ntawm kev tshwj xeeb txwv tsis pub endonucleases thiab txuam DNA sequences tso.
Qhov twg ntawm cov kev txwv hauv qab no endonuclease yuav txiav pBR322 plasmid ntawm TetR tetracycline resistance gene?
Kev txwv enzyme BamHI yuav tsum tau siv los ua qhov chaw txwv rau qhov enzyme no nyob hauv cov noob coding rau tetracycline tsis kam.
Vim li cas E coli tiv taus tetracycline?
coli (4, 7), uas qhia tias kev tiv thaiv tau raug xaiv los ntawm cov neeg sawv cev ntawm cov txiaj ntsig E. coli, thaum kho lwm yam kab mob hauv tib neeg lossis tsiaj. Cov kab mob tsis kam mus rau tetracycline feem ntau yog kho los ntawm zog-nyob twj ywm ntawm tetracycline tawm ntawm cov kab mob cell.
