2024 Tus sau: Elizabeth Oswald | [email protected]. Kawg hloov kho: 2024-01-13 00:07
Teb: Lub ligation ntawm alien DNA yog nqa tawm ntawm qhov chaw txwv tam sim no hauv ib qho ntawm ob hom tshuaj tiv thaiv kab mob. Thaum ib tug txawv teb chaws DNA yog ligated nyob rau hauv Sal I qhov chaw ntawm tetracycline tsis kam gene nyob rau hauv lub vector pBR322, cov recombinant plasmids yuav poob tetracycline tsis kam vim yog insertion ntawm txawv teb chaws DNA.
Yuav ua li cas tus neeg txawv teb chaws DNA nce nkag mus rau hauv cov nroj tsuag ntawm tes los ntawm txoj kev biolistic?
Nyob rau hauv txoj kev bioloistic, ib tug neeg txawv teb chaws DNA yog ua rau nkag mus rau hauv cov nroj tsuag hlwb los ntawm high-speed bombardment ntawm kub los yog tungsten hais. Nyob rau hauv cov txheej txheem no, cov DNA-coated particles yog propelled los ntawm ib tug pressurized phom hu ua rab phom noob los qhia cov neeg txawv teb chaws DNA rau hauv cov nroj tsuag hlwb.
Thaum tus neeg txawv teb chaws DNA yog ligated hauv tetracycline tsis kam gene Cov recombinant?
Thaum tus neeg txawv teb chaws noob ligated ntawm Sal I qhov chaw ntawm tetracycline tsis kam gene hauv vector pBR322 cov recombinant poob tetracycline tsis kam vim rau ntxig rau ntawm txawv teb chaws DNA.
Yuav ua li cas yog tias cov noob paj tau tso rau ntawm qhov chaw txwv ntawm BamHI hauv cloning vector pBR322?
Thaum ib qho ntxig ntxiv rau ntawm qhov chaw paub BamHI cov noob rau tetracycline tsis ua haujlwm yuav tsis ua haujlwm thiab cov kab mob sib txuas nrog plasmid pBR322 uas muaj DNA ntxig ntawm BamHI poob tetracycline tsis kam..
Koj xav li cas yuav tshwm sim thaum tus neeg txawv teb chaws noob ligated ntawm PVUI qhov chaw ntawm plasmidpBR322 ntawm E coli?
Thaum ib tus neeg txawv teb chaws yog ligated ntawm Pvu kuv qhov chaw ntawm ampicillin resistance gene nyob rau hauv vector pBR322 the recombinant plasmids poob ampicillin tsis kam vim yog ntxig rau txawv teb chaws DNA.
Pom zoo:
Thaum twg dna replicates dab tsi txiav thiab yuav ua li cas?
Qhov tshwm sim ntawm DNA rov ua dua yog ob DNA molecules muaj ib qho tshiab thiab ib qho qub saw ntawm nucleotides. Qhov no yog vim li cas DNA replication yog piav raws li ib tug conservative, ib nrab ntawm cov saw yog ib feem ntawm tus thawj DNA molecule, ib nrab yog brand tshiab.
Thaum twg siv i.e. thiab thaum twg siv xws li?
I.e. yog ib qho luv luv rau kab lus id est, uas txhais tau tias "uas yog." I.e. yog siv los rov hais dua ib yam dab tsi uas tau hais yav dhau los txhawm rau kom meej nws lub ntsiab lus. E.g. luv luv rau exempli gratia, uas txhais tau tias "
Thaum twg siv polyacrylamide thiab thaum twg agarose?
Agarose gels siv nrog DNA, vim qhov loj ntawm biomolecules (DNA feem ntau yog ntau txhiab kDa). Rau cov protein gels, polyacrylamide muab daws teeb meem zoo, raws li qhov me me me (50 kDa yog qhov raug) zoo dua rau qhov sib txawv ntawm cov gel.
Lub sijhawm thyroidectomy superior thyroid artery yog ligated ntawm?
Vim qhov hloov pauv tsis zoo ntawm cov thyroid caj pas tsis zoo thiab RLN RLN Kev raug mob rau cov hlab ntsha rov qab tuaj yeem ua rautsis muaj suab nrov (qhoarseness) lossis poob suab (aphonia) thiab ua rau muaj teeb meem hauv kev ua pa. Kev raug mob rau cov hlab ntsha tuaj yeem ua rau tuag tes tuag taw cov leeg tom qab cricoarytenoid ntawm tib sab.
Thaum twg mitochondrial dna siv?
Ib qho pej xeem siv rau mtDNA hauv kev kuaj DNA yog hauv kev txiav txim siab poj koob yawm txwv. Vim hais tias mtDNA tsis hloov pauv sai npaum li cov DNA DNA, thiab vim nws tsis sib xyaw nrog leej txiv (niam txiv) DNA, nws tso cov ntaub ntawv qhia meej dua ntawm cov poj koob yawm txwv nyob deb - txawm tias tsuas yog los ntawm leej niam '(niam poj koob yawm txwv).
